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The induction in vitro of adventitious shoots in Rosa

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Summary

Adventitious shoots were formed on excised leaves, roots and callus of Rosa persica x xanthina and on excised leaves of R. laevigata and R. wichuraiana on culture media that included BAP and NAA as growth regulators. Shoots formed freely on freshly cultured callus of R. persica x xanthina but their production declined in successive cultures and ceased after twelve weeks. Transplantation to soil was improved by rooting plantlets in cellulose plugs in vitro and transferring plantlets to soil while still in the plugs.

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References

  • Barg, R. & N. Umiel, 1977. Effects of sugar concentration on growth, greening and shoot formation in callus cultures from 4 genetic lines of tobacco. Z Pflanzenphysiol 81: 161–166.

    Google Scholar 

  • Bressan, P.H., Y.-J. Kim & P.M. Hasegawa, 1980. Plant regeneration from cultured rose shoot tips. Hort Sci 15: 432.

    Google Scholar 

  • Bressan, P.H., Y.-J. Kim, S.E. Hyndman, P.M. Hasegawa & R.A. Bressan, 1982. Factors affecting in vitro propagation of rose. J Am Soc Hort Sci 107: 979–990.

    Google Scholar 

  • Davies, D.R., 1980. Rapid propagation of roses in vitro. Scientia Hort 13: 385–389.

    Google Scholar 

  • De Fossard, R.A. & E.C.M. Lee, 1974. A broad spectrum tissue culture experiment with tobacco (Nicotiana tabacum) pith tissue callus. Physiol Plant 30: 125–130.

    Google Scholar 

  • George, E.F. & P.D. Sherrington, 1984. Plant propagation by tissue culture, Exegetics Ltd., Basingstoke, England. 709p.

    Google Scholar 

  • Hasegawa, P.M., 1979. In vitro propagation of rose. Hort Sci 14: 610–612.

    Google Scholar 

  • Hasegawa, P.M., 1980. Factors affecting shoot and root initiation from cultured rose shoot tips. J Am Soc Hort Sci 105: 216–220.

    Google Scholar 

  • Hill, G., 1967. Morphogenesis of shoot primordia in cultured stem tissue of a garden rose. Nature 216: 596–597.

    Google Scholar 

  • Hyndman, S.E., P.M. Hasegawa & R.A. Bressan, 1982. Stimulation of root initiation from cultured rose shoots through the use of reduced concentrations of mineral salts. Hort Sci 17: 82–84.

    Google Scholar 

  • James, D.J., A.J. Passey & S.B. Malhotra, 1984. Organogenesis in callus derived from stem and leaf tissues of apple and cherry rootstocks. Plant Cell Tiss Organ Cult 3: 333–341.

    Google Scholar 

  • Khosh-Khui, M. & K.C. Sink, 1982. Rooting enhancement of Rosa hybrida for tissue culture propagation. Scientia Hort 17: 361–370.

    Google Scholar 

  • Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco callus. Physiol Plant 15: 473–497.

    Google Scholar 

  • Peterson, R.L., 1975. The initiation and development of root buds. In: J.G. Torrey & D.T. Clarkson (Eds.), The development and fuction of roots. Academic Press, London & New York: 125–161.

    Google Scholar 

  • Sass, J.E., 1958. Botanical Microtechnique, 3rd ed, Iowa State College Press, Ames, IA. 228 p.

    Google Scholar 

  • Skirvin, R.M. & M.C. Chu, 1979. In vitro propagation of ‘Forever Yours’ rose. Hort Sci 14: 608–610.

    Google Scholar 

  • Tabaeezadeh, Z. & M. Khosh-Khui, 1981. Anther culture of Rosa. Scientia Hort 15: 61–66.

    Google Scholar 

  • Thorpe, T.A., 1978. Regulation of organogenesis in vitro. In: K.W. Hughes, R. Henke & M. Constantin (Eds.), Propagation of higher plants through tissue culture. Tech Info Centre, US Department of Energy, Springfield, VA: 49–58.

    Google Scholar 

  • Thorpe, T.A. & E.J. Laishley, 1973. Glucose oxidation during shoot initiation in tobacco. J exp Bot 24: 1082–1089.

    Google Scholar 

  • Thorpe, T.A. & D.D. Meier 1974a. Starch metabolism in shoot forming tobacco callus. J exp Bot 25: 288–294.

    Google Scholar 

  • Thorpe, T.A. & D.D. Meier, 1974b. Carbohydrate availability and shoot formation in tobacco callus cultures. Physiol Plant 30: 77–81.

    Google Scholar 

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Lloyd, D., Roberts, A.V. & Short, K.C. The induction in vitro of adventitious shoots in Rosa . Euphytica 37, 31–36 (1988). https://doi.org/10.1007/BF00037220

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  • DOI: https://doi.org/10.1007/BF00037220

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