Summary
Interspecific hybrids have been obtained in an incompatible cross between Glycine max and G. tomentella through the in vitro culture of hybrid embryos. The percentage of successful pod setting in the crosses averaged 12.8% but there were marked differences depending on the soybean cultivar used as the female parent. Hybrid embryos at globular to heart stages were extracted from the embryo sac 15–25 days after pollination and cultured in vitro. Hybrid plants were successfully obtained by culturing the embryos on B5 medium supplemented with 0.1 mg/l IBA followed by culture on B5 medium supplemented with 0.1 mg/l TBA plus 0.25 mg/l 2-iP. The F1 plants resembled the wild male parent in growth form, but had an intermediate leaf shape between that of the parents.
Similar content being viewed by others
References
Barwale, U.B., H.R. Kerns & J.M. Widholm, 1986. Plant regeneration from callus cultures of several Glycine max (L.) Merr. genotypes via embryogenesis and organogenesis. Planta 167: 473–481.
Broue, P., J. Douglass, J.P. Grace & D.R. Marshall, 1982. Interspecific hybridization of soybean and perennial Glycine species indigenous to Australia via embryo culture. Euphytica 31: 715–724.
Chung, G.H., 1988. Intraspecific hybridization through embryo culture and Agrobacterium mediated transformation in the Genus Glycine. Ph.D. Thesis, Gyeongsang Natl. Univ., Korea.
Gamborg, O.L., R.A. Miller & K. Ojima, 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158.
Hammatt, N. & M.R. Davey, 1987. Somatic embryogenesis and plant regeneration from cultured zygotic embryos of soybean (Glycine max L. Merr.). J. Plant Physiol. 128: 219–226.
Ladizinsky, G., C.A. Newell & T. Hymowitz, 1979. Wide crosses in soybeans: prospects and limitations. Euphytica 28: 421–423.
Newell, C.A. & T. Hymowitz, 1982. Successful wide hybridization between the soybean and a wild perennial relative, G. tomentella Hayata. Crop Sci. 22: 1062–1065.
Newell, C.A., X. Delannay & M.E. Edge, 1987. Interspecific hybrids between the soybean and wild perennial relatives. J. Heredity 78: 301–306.
Palmer, R.J. & H.H. Hadley, 1968. Interspecific hybridization in Glycine, subgenus Leptocyanus. Crop Sci. 66: 557–563.
Ranch, J.P., L. Oglesby & A.C. Zielinsky, 1985. Plant regeneration from embryo-derived tissue cultures of soybeans. In Vitro 21: 653–658.
Sakai, T. & N. Kaizuma, 1985. Hybrid embryo formation in an intersubgeneric cross of soybean (Glycine max Merril) with a wild relative (G. tomentella Hayata). Japan J. Breed. 35: 363–374.
Singh, R.J. & T. Hymowitz, 1985. An interspecific hybrid between Glycine tomentella Hayata and the soybean, G. max (L.) Merr. Euphytica 34: 187–192.
Stalker, H.T., 1980. Utilization of wild species for crop improvement. Adv. Agron. 33: 112–147.
Tilton, V.R. & S.H. Russel, 1984. In vitro cultures of immature soybean embryo. J. Plant Physiol. 115: 191–200.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chung, G.H., Kim, J.H. Production of interspecific hybrids between Glycine max and G. tomentella through embryo culture. Euphytica 48, 97–101 (1990). https://doi.org/10.1007/BF00037186
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00037186