Plant Molecular Biology

, Volume 17, Issue 5, pp 1105–1109 | Cite as

Universal primers for amplification of three non-coding regions of chloroplast DNA

  • Pierre Taberlet
  • Ludovic Gielly
  • Guy Pautou
  • Jean Bouvet
Update Section Short Communication


Six primers for the amplification of three non-coding regions of chloroplast DNA via the polymerase chain reaction (PCR) have been designed. In order to find out whether these primers were universal, we used them in an attempt to amplify DNA from various plant species. The primers worked for most species tested including algae, bryophytes, pteridophytes, gymnosperms and angiosperms. The fact that they amplify chloroplast DNA non-coding regions over a wide taxonomic range means that these primers may be used to study the population biology (in supplying markers) and evolution (inter- and probably intraspecific phylogenies) of plants.

Key words

chloroplast DNA non-coding region polymerase chain reaction primer 


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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Pierre Taberlet
    • 1
  • Ludovic Gielly
    • 1
  • Guy Pautou
    • 1
  • Jean Bouvet
    • 1
  1. 1.Laboratoire de Biologie et d'Evolution des PopulationsUniversité Joseph FourierGrenoble CedexFrance

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