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Novel DNA structures resulting from dTam3 excision in tobacco

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Abstract

A Tam3 two-element system has been designed by combining an immobilized Tam3 element with a non-autonomous dTam3 element inserted into the HPT gene. The phenotypic assay employed, restored hygromycin resistance, indicated thattrans-activation of the non-autonomous dTam3 element occurred. Molecular analyses of the excision sites revealed that the ends of the dTam3 element remain in the empty donor sites. The predominant consequence of this type of excision appears to be that excised fragments fail to re-integrate into the tobacco genome. Only one case of dTam3 re-integration could be detected. The ends of this element had been degraded upon integration into the tobacco genome. Either the altered structure of the Tam3 derivatives or tobacco host factors are influencing thetrans-activation of a dTam3 element, resulting in aberrant excision.

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Author notes

  1. Michel A. Haring

    Present address: Molecular Cell Biology, University of Amsterdam, Kruislaan 318, 1098 SM, Amsterdam, Netherlands

Authors and Affiliations

  1. Dept. of Genetics, Free University, de Boelelaan 1087, 1081 HV, Amsterdam, Netherlands

    Michel A. Haring, Marianne J. Teeuwen-de Vroomen, Gerjan S. Leuring, H. John J. Nijkamp & Jacques Hille

  2. The Sainsbury Laboratory, John Innes Institute, Colney Lane, NR4 7UH, Norwich, United Kingdom

    Steve Scofield

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  1. Michel A. Haring
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  5. H. John J. Nijkamp
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  6. Jacques Hille
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Haring, M.A., Scofield, S., Teeuwen-de Vroomen, M.J. et al. Novel DNA structures resulting from dTam3 excision in tobacco. Plant Mol Biol 17, 995–1004 (1991). https://doi.org/10.1007/BF00037139

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  • DOI: https://doi.org/10.1007/BF00037139

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