Summary
Determination of pedigree by laboratory means can provide an initial and rapid check of hybrid identity in studies designed to compare genotypes. In order to test the accuracy of such determinations, isozymic and chromatographic data were used in an attempt to ascribe pedigrees to 25 hybrids that were identified only by a letter code from a list of 30 inbred lines. The pedigrees of 9 single-cross hybrids were correctly deduced as was the direction in which each cross was made. The pedigrees of 2 hybrids could only be determined ambiguously, however these hybrids were reciprocals and the biochemical data were unable to distinguish between 2 related candidate inbred lines. Pedigrees of 3 hybrids were either incompletely determined or could not be determined from the list of 30 candidate inbred lines and were thus correctly identified as non-Pioneer® brand hybrids. Pedigrees of 9 3-way hybrids could be determined accurately but the pedigrees of 2 non-Pioneer® brand hybrids could not be deduced completely. The ability to correctly ascribe pedigrees of hybrids shows that these data provide a rapid means of determining whether additional field and laboratory tests would be warranted in tests designed to compare genotypic similarity of hybrids.
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Smith, J.S.C. Identification of pedigrees of hybrid maize (Zea mays L.) cultivars by isozyme electrophoresis and reversed-phase high-performance liquid chromatography. Euphytica 39, 199–205 (1988). https://doi.org/10.1007/BF00037096
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DOI: https://doi.org/10.1007/BF00037096