Abstract
Mismatch-specific enzymatic activity was sought for in nuclei from normal and transformed plant cells originating from tobacco (Nicotiana tabacum) callus and crown gall tumor induced by Agrobacterium tumefaciens. The specific enzymatic activity was assayed with substrates derived from synthetic oligonucleotides (19-mer sequences corresponding to the human K-ras gene). Single-base changes in the middle of the sequence were the basis for creating heteroduplexes with all eight mismatches. Homo- and heteroduplexes were ligated in a size ladder and used as substrates. We detected mismatch-specific DNA breakdown and determined basic requirements for the reations. Kinetic analysis indicates the following reactivity order of preference: C : A=C : C=C : T>G : T∼A : A∼G : A∼G : G∼T : T>>G : C. It can be said now that specific mismatch recognition and repair activities have been detected in all kingdoms of living species.
Similar content being viewed by others
References
Barbacid, M: ras Genes. Ann Rev Biochem 56: 779–827 (1987).
Brown, TC, Jiricny, J: Different base/base mispairs are corrected with different efficiencies and specificities in monkey kidney cells. Cell 54: 705–711 (1988).
Creusot, F, Acs, G, Christmans, KJ: Inhibition of DNA methyltransferase and induction of Friend erythroleukemia cell differentiation by 5-azacytidine and 5-aza-2′-deoxycytidine. J Biol Chem 257: 2041–2048 (1982).
De, Vos, G, De, Beuckeleer, M, Van, Montagu, M, Schell, J: Restriction endonuclease mapping of the octopine tumorinducing plasmid pTiAch5 of Agrobacterium tumefaciens. Plasmid 6: 249–253 (1981).
Dimitrijevic B, Sunjevaric I, Cerovic G, Bozin D, Radman M: Recognition of base pair mismatches at the site of oncogenic K-ras mutations by a mammalian cell-free extract. Cancer Res, submitted for publication (1991).
Dohet, C, Wagner, R, Radman, M: Repair of defined single base-pair mismatches in Escherichia coli. Proc Natl Acad Sci USA 82: 503–505 (1985).
Gasc, AM, Sicard, AM, Claverys, JP: Repair of single- and multiple-substitution mismatches during recombination in Streptococcus pneumoniae. Genetics 121: 29–36 (1989).
Holmes, J, Clark, S, Modrich, P: Strand-specific mismatch correction in nuclear extracts of human and Drosophila melanogaster cell lines. Proc Natl Acad Sci USA 87: 5837–5841 (1990).
Jiricny, J, Su, SS, Wood, SG, Modrich, P: Mismatchcontaining oligonucleotide duplexes bound by the Escherichia coli mutS-encoded protein. Nucl Acid Res 16: 7843–7853 (1988).
Jones, M, Wagner, R, Radman, M: Repair of a mismatch is influenced by the base composition of the surrounding nucleotide sequence. Genetics 115: 605–610 (1987).
Jones, M, Wagner, R, Radman, M: Mismatch repair of deaminated 5-methyl-cytosine. J Mol Biol 194: 155–159 (1987).
Kramer, B, Kramer, W, Williamson, MS, Fogel, S: Heteroduplex DNA correction in Saccharomyces cerevisiae is mismatch specific and requires functional PMS genes. Mol Cell Biol 9: 4432–4440 (1989).
McMaster, GK, Carmichael, GG: Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci USA 74: 4835–4838 (1977).
Meselson, M: Methyl-directed repair of DNA mismatches. In: Low, KB (ed), Recombination of Genetic Material, pp. 91–114. Academic Press, New York (1988).
Modrich, P: DNA mismatch correction. Annu Rev Biochem 56: 435–466 (1987).
Murashige, T, Skoog, F: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15: 473–497 (1962).
Radman, M, Wagner, R: Mismatch repair in Escherichia coli. Annu Rev Genet 20: 523–538 (1986).
Radman, M: Mismatch repair and recombination. In: Kucherlapati, R, Smith, GR (eds), Genetic Recombination, pp. 169–192. American Society for Microbiology, Washington DC (1988).
Schaaper, RM, Dunn, RL: Spectra of spontaneous mutations in Escherichia coli strains defective in mismatch correction: The nature of in vivo DNA replication errors. Proc Natl Acad Sci USA 84: 6220–6224 (1987).
Soyfer, VN: DNA damage, repair and mutagenesis in higher plants. Israel J Bot 36: 1–14 (1987).
Spelsberg, TC, Sarkissian, IV: Isolation and analysis of the proteins of plant nuclei from two stages of differentiation of Phaseolus vulgaris. Phytochemistry 9: 1385–1391 (1970).
Su, SS, Lahue, RS, Au, KG, Modrich, P: Mispair specificity of methyl-directed mismatch correction in vitro. J Biol Chem 263: 6829–6835 (1988).
Varlet, I, Radman, M, Brooks, P: DNA mismatch repair in Xenopus eggs extracts: Repair efficiency and DNA repair synthesis for all single base-pair mismatches. Proc Natl Acad Sci USA 87: 7883–7887 (1990).
Veleminsky, J, Gichner, T: DNA repair in mutagen-injured plants. Mutation Res 55: 71–84 (1978).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cerovic, G., Bozin, D. & Dimitrijevic, B. Mismatch-specific DNA breakdown in nuclear extract from tobacco (Nicotiana tabacum) callus. Plant Mol Biol 17, 887–894 (1991). https://doi.org/10.1007/BF00037069
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00037069