Abstract
A full-length cDNA encoding glutamine synthetase was isolated from a λgt11 library constructed from the poly(A)+ RNA isolated from lettuce seeds incubated under red light. The nucleotide sequence of the cDNA and the deduced sequence of amino acids showed a high degree of homology to those of the cytosol-type glutamine synthetase from other plants. Northern and dot-blot analyses of poly(A)+ RNA extracted from the seeds incubated under various light conditions showed that the activation of the gene for cytosolic glutamine-synthetase during imbibition of lettuce seeds is directly or indirectly regulated by phytochrome.
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Abbreviations
- GS:
-
glutamine synthetase
- GA:
-
gibberellin
References
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Sakamoto, A., Takeba, G., Shibata, D. et al. Phytochrome-mediated activation of the gene for cytosolic glutamine-synthetase (GS1) during imbibition of photosensitive lettuce seeds. Plant Mol Biol 15, 317–323 (1990). https://doi.org/10.1007/BF00036917
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DOI: https://doi.org/10.1007/BF00036917