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In vitro organogenesis and plant regeneration from leaves of Solanum candidum Lindl, S. quitoense Lam. (naranjilla) and S. sessiliflorum Dunal

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Abstract

Adventitious shoots and roots were regenerated from leaf segments of 3 Solanum species: S. candidum Lindl., S. quitoense Lam. and S. sessiliflorum Dunal. Leaf explants differentiated shoots on modified MS medium supplemented with 23–163 μM kinetin and 0–5.7 µM indoleacetic acid (IAA). Excised shoots were induced to form roots by transfer to media with benzyladenine (BA) and naphthaleneacetic acid (NAA) at 0.09 and 0.11 µM respectively for S. quitoense and 0.01 µM NAA for S. candidum and S. sessiliflorum. Adventitious roots were produced directly from leaf explants with 0–140 µM kinetin and 0–5.7 µM IAA in combination. Rooted plants were successfully established in the greenhouse.

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Hendrix, R.C., Litz, R.E. & Kirchoff, B.K. In vitro organogenesis and plant regeneration from leaves of Solanum candidum Lindl, S. quitoense Lam. (naranjilla) and S. sessiliflorum Dunal. Plant Cell Tiss Organ Cult 11, 67–73 (1987). https://doi.org/10.1007/BF00036577

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  • DOI: https://doi.org/10.1007/BF00036577

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