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In vitro propagation of Amaryllis belladonna

Plant Cell, Tissue and Organ Culture volume 31, pages 179–184 (1992)Cite this article

Abstract

Amaryllis belladonna L. plants were multiplied successfully by means of tissue culture techniques. Different plant parts were tested as explant material, but plantlets could only be generated from the twin-scales and immature scapes. These in vitro-formed plantlets were divided into four parts and used for further multiplication. The twin-scale explants had the highest multiplication rate when a medium with 22.2 μM benzyladenine and 0.54 μM naphthaleneacetic acid was used. The sucrose concentration played an important role in the initiation of new plantlets, and the best results were obtained when a sucrose concentration of 2–3% was used. Anatomical observations were made during the initiation of the new plantlets.

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Abbreviations

BA:

benzyladenine

NAA:

naphthaleneacetic acid

Benomyl:

(methyl [1-[(butylamino) carbonyl]-1H-benzimidazol-2-yl] carbamate)

Folpet:

(2-[(trichloromethyl)thio]-H-isoindole-1,3(2H)-dione phthalimide(I))

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Authors and Affiliations

  1. Vegetable and Ornamental Plant Research Institute, Private Bag X293, 0001, Pretoria, Republic of South Africa

    M. H. De Bruyn

  2. Research Centre for Plant Biotechnology, Private Bag X293, 0001, Pretoria, USA

    D. I. Ferreira & M. M. Slabbert

  3. Department of Botany, Rand Afrikaans University, P.O. Box 524, 2000, Johannesburg, RSA

    J. Pretorius

Authors
  1. M. H. De Bruyn
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  2. D. I. Ferreira
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  3. M. M. Slabbert
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  4. J. Pretorius
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De Bruyn, M.H., Ferreira, D.I., Slabbert, M.M. et al. In vitro propagation of Amaryllis belladonna . Plant Cell Tiss Organ Cult 31, 179–184 (1992). https://doi.org/10.1007/BF00036221

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  • DOI: https://doi.org/10.1007/BF00036221

Key words

  • Belladonna lily
  • Cape belladonna
  • March lily
  • micropropagation
  • sucrose
  • tissue culture
  • twin-scales