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Elimination of ornithogalum mosaic virus in the Ornithogalum cv. Rogel through meristem tip culture and chemotherapy

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Abstract

Virus-indexed Ornithogalum ev. Rojel plants were produced by eliminating Ornithogalum mosaic virus (OMV) through meristem-tip culture. The best plantlet regeneration was obtained from meristems derived from adventitious buds which developed on leaf explants taken from mother plants at the flowering stage. Acyclovir had no effect as an anti-viral compound on plantlet regeneration or virus elimination. Adenine arabinoside retarded plantlet development at concentrations of 10 mg l-1 and higher, while 5.0 mg l-1 suppressed the virus concentration beneath a detectable level in young plants. All the mature plants, however, tested positively for the presence of OMV.

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Abbreviations

BA:

6-benzyladenine

NAA:

α-naphthaleneacetic acid

OMV:

Ornithogalum mosaic virus

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Authors and Affiliations

  1. Vegetable and Ornamental Plant Research Institute, Private Bag X293, 0001, Pretoria, Republic of South Africa

    Bela M. Vcelar & Josephina G. Niederwieser

  2. Plant Biotechnology Research Centre, Private Bag X293, 0001, Pretoria, Republic of South Africa

    David I. Ferreira

Authors
  1. Bela M. Vcelar
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  2. David I. Ferreira
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  3. Josephina G. Niederwieser
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Vcelar, B.M., Ferreira, D.I. & Niederwieser, J.G. Elimination of ornithogalum mosaic virus in the Ornithogalum cv. Rogel through meristem tip culture and chemotherapy. Plant Cell Tiss Organ Cult 29, 51–55 (1992). https://doi.org/10.1007/BF00036146

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  • DOI: https://doi.org/10.1007/BF00036146

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