Abstract
Significant differences in somatic embryogenesis from melon seeds were observed among 18 cultivars; especially, cultivars ‘Earl's Favorite’ and ‘Barnett’ which produced a large number of somatic embryos. F1 seeds were obtained by reciprocal crosses between cultivars. Some lines produced a large number of somatic embryos whereas others showed no or poor embryogenic response. Most of the F1 seeds formed somatic embryos. The frequency of somatic embryogenesis decreased as compared to the parents with the highest potential. Transfer of the frequency of somatic embryogenesis from superior responding cultivars to inferior cultivars was proved. It was difficult to determine the mode of inheritance of somatic embryogenesis because there was a large variation in the range of somatic embryogenesis from F2 seeds, and cytoplasmic effect was recognized in certain combinations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- BAP:
-
6-benzylaminopurine
References
AmmiratoPV (1989) Recent progress in somatic embryogenesis. Newsl. Int. Assoc. Plant Tissue Cult. 57: 2–16
ArzaniAS, ShimonishiK, AsanoY & OosawaK (1989) Somatic embryogenesis and organogenesis from in vitro seed culture of melon. Japan. J. Breed. 39 (supple. 2): 138–139
BrownDCW (1988) Germplasm determination of in vitro somatic embryogenesis in alfalfa. HortScience 23: 526–531
ChuCC, WangCC, SunCS, YinKC & ChuCY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scientia Sinica 18: 659–668
DirksR & vanBuggenumM (1989) In vitro plant regeneration from leaf and cotyledon explants of Cucumis melo L. Plant Cell Rep. 7: 626–627
FaheyJW, ReedJN, ReaddyTL & PaceGM (1986) Somatic embryogenesis from three commercially important inbreds of Zea mays. Plant Cell Rep. 5: 35–38
Hayashi E & Ooshima K (1989) Induction of embryogenic calli and plant regeneration from shoot apices in melon. Abstracts 11th Symposium of Plant Tissue Cult. (p. 49). Japan
KubotaT & OosawaK (1987) Effective conditions and varietal difference on induction of somatic embryos in suspension culture of melon. Japan. J. Breed. 37 (Supple. 1): 138–139
LazarMD, BanezigerPS & SchaefferCW (1984) Combining abilities and heritability of callus formation and plantlet regeneration in wheat (Triticum aestivum L.) anther culture. Theor. Appl. Genet. 68: 131–134
LazarMD, SchaefferGW & BaenzgerPS (1990) The effects of interactions of culture environment with genotype on wheat (Triticum aestivum L.) anther culture response. Plant Cell Rep. 8: 525–529
MathiasRJ, FukuiK & LawCN (1986) Cytoplasmic effects on the tissue culture response of wheat (Triticum aestivum L). callus. Theor. Appl. Genet. 72: 70–75
OridateT & OosawaK (1986) Somatic embryogenesis and plant regeneration from suspension callus culture in melon. Japan. J. Breed. 36: 424–428
PunjaZK, AbbasN, SarmentoGG & TangFA (1990) Regeneration of Cucumis sativus var. sativum and C. sativus var. hardwickii, C. melo and C. metuliferus from explants through somatic embryogenesis and organogenesis. Plant Cell Tiss. Org. Cult. 21: 93–102
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Oridate, T., Atsumi, H., Ito, S. et al. Genetic difference in somatic embryogenesis from seeds in melon (Cucumis melo L.). Plant Cell Tiss Organ Cult 29, 27–30 (1992). https://doi.org/10.1007/BF00036142
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00036142