Abstract
Micropropagation of the actinorhizal plant Comptonia peregrina of the Myricaceae was achieved successfully by the induction of root buds in excised root culture with cytokinin (1.0 μM benzyladenine). Excised root segments with initiated root buds were subcultured in Woody Plant Medium (WPM) lacking growth regulators, developing extensive callus which subsequently gave rise to multiple adventitious buds. Shoot elongation was facilitated by transfer of calluses to more aerated conditions. Root initiation was induced on shoots by brief treatment with auxin (<1 μM indolebutyric acid) and transfer to WPM for plantlet development. Controlled light and aeration in liquid medium were critical conditions for successful micropropagation.
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References
Barghchi M (1988) Micropropagation of Alnus cordata (Loisel.) Loisel. Plant Cell Tiss. Org. Cult. 15: 233–244
Bertrand LJ & Lalonde M (1985) In vitro propagation and nodulation by Frankia of the actinorhizal Russian olive (Elaeagnus angustifolia L.). Plant and Soil 87: 143–152
Del Tredici P & Torrey JG (1977) On the germination of seeds of Comptonia peregrina, the sweet fern. Bot. Gaz. 137: 262–268
Fessenden RJ (1976) Use of actinorhizal plants for land reclamation and amenity planting in the USA and Canada. In: Gordon JC, Wheeler CT & Perry DA (Eds) Symbiotic Nitrogen Fixation in the Management of Temperate Forests (pp 403–419). Oregon State University, Corvallis, OR
Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158
Gamborg OL & Shyluk JP (1976) Tissue culture, protoplasts and morphogenesis in flax. Bot. Gaz. 137: 301–306
Goforth PL & Torrey JG (1977) The development of isolated roots of Comptonia peregrina (Myricaceae) in culture. Amer. J. Bot. 64: 476–482
Link GKK & Eggers V (1946) Mode, site and time of initiation of hypocotyledonary bud primordium in Linum usitatissimum L. Bot. Gaz. 107: 441–454
Lloyd G & McCown B (1981) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Comb. Proc. Int. Plant Prop. Soc. 30: 421–426
Montpetit D & Lalonde M (1988) In vitro propagation and subsequent nodulation of the actinorhizal Hippophae rhamnoides L. Plant Cell Tiss. Org. Cult. 15: 189–199
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Perinet P & Lalonde M (1983) In vitro propagation and nodulation of the actinorhizal host plant Alnus glutinosa (L.) Gaertn. Plant Sci. Lett. 29: 9–17
Perinet P, Vallee G & Tremblay FM (1988) In vitro propagation of mature trees of Alnus incana (L.) Moench. Plant Cell Tiss. Org. Cult. 15: 85–89
Tremblay FM & Lalonde M (1984) Requirements for in vitro propagation of seven nitrogen-fixing Alnus species. Plant Cell Tiss. Org. Cult. 3: 189–199
Tremblay FM, Nesme X & Lalonde M (1984) Selection and micropropagation of nodulating and non-nodulating clones of Alnus crispa (Ait.) Pursh. Plant and Soil 78: 171–178
Zhan XC, Jones DA & Kerr A (1989) Regeneration of shoots on root explants of flax. Ann. Bot. 63: 297–299
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Louis, I., Torrey, J.G. In vitro clonal multiplication of the actinorhizal plant Comptonia peregrina . Plant Cell Tiss Organ Cult 26, 89–96 (1991). https://doi.org/10.1007/BF00036111
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DOI: https://doi.org/10.1007/BF00036111