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Micropropagation of Withania somnifera from germinating seeds and shoot tips

Plant Cell, Tissue and Organ Culture volume 26, pages 71–73 (1991)Cite this article

Abstract

Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. using low concentrations of 6-benzyladenine (BA), viz. 2.2, 4.4 and 8.9 μM. Maximum number of shoots were obtained when 2.3 μM 2,4-dichlorophenoxyacetic acid (2,4-D) or 2.5 μM indolebutyric acid (IBA) was added to medium containing 4.4 μM BA during initiation of shoot multiplication, but not when added later. Direct multiple shoot initiation was also obtained from germinating seeds in the presence of BA alone. Rooting was successful in excised shoots grown on growth regulator-free MS medium. Rooted shoots were successfully established in soil in a greenhouse.

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Authors and Affiliations

  1. Centre of Advanced Study, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, 700 019, Calcutta, India

    Jayanti Sen & A. K. Sharma

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  1. Jayanti Sen
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  2. A. K. Sharma
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Sen, J., Sharma, A.K. Micropropagation of Withania somnifera from germinating seeds and shoot tips. Plant Cell Tiss Organ Cult 26, 71–73 (1991). https://doi.org/10.1007/BF00036108

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  • DOI: https://doi.org/10.1007/BF00036108

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