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Assessment of liquid culture procedures for in vitro propagation of Rheum emodi

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Abstract

Micropropagation of an endangered Indian medicinal plant, Rheum emodi Wall., was achieved on Murashige and Skoog's medium using different liquid culture procedures. Liquid static (submerged, semi-submerged and with filter paper bridge) and shake (80 and 120 rpm) culture procedures were assessed for their effects on growth and multiplication rates. Best results were obtained using liquid shake cultures, which resulted in 50% reduction in medium requirement, 37.5% reduction in time and 1.5–2.2 fold increase in growth and multiplication rate. Liquid culture-raised plantlets facilitated easy transplantation and 90–95% survived transfer to potting mix in glasshouse.

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Abbreviations

BA:

6-benzyladenine

IBA:

indole-3-butyric acid

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Author notes

  1. Nand Lal

    Present address: Plant Tissue Culture Laboratory, G.S. Sugarcane Breeding and Research Institute, Seorahi, P.O. Tamkuhi Raj, 274407, Deoria, India

Authors and Affiliations

  1. Division of Plant Tissue Culture, Central Institute of Medicinal and Aromatic Plants (CIMAP), RSM Nagar, P.B. No. 1, 226016, Lucknow, (India)

    Nand Lal & Paramvir Singh Ahuja

Authors
  1. Nand Lal
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  2. Paramvir Singh Ahuja
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Lal, N., Ahuja, P.S. Assessment of liquid culture procedures for in vitro propagation of Rheum emodi . Plant Cell Tiss Organ Cult 34, 223–226 (1993). https://doi.org/10.1007/BF00036106

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  • DOI: https://doi.org/10.1007/BF00036106

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