Abstract
Four tronchuda (Brassica oleracea var. tronchuda Bailey) cultivars were tested for their ability to regenerate in vitro on Murashige & Skoog (MS) medium supplemented with 3 different combinations of α-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP). Explants were either axillary bud-free whole cotyledons or hypocotyls from 7-day-old darkgrown seedlings. The ability to regenerate varied by cultivars, explants and the concentration of growth regulators. Hypocotyl explants of all 4 cultivars, and cotyledon explants of 2 cultivars, developed plantlets within 4 weeks. Hypocotyl explants produced more shoots than cotyledons. Cotyledon explants produced more roots than hypocotyls. Best shoot regeneration was on MS medium supplemented with 2 mgl-1 BAP and 0.1 mgl-1 NAA. ‘Portuguesa’ produced the most shoots. Some regenerants varied in leaf shape and phyllotaxy.
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Abbreviations
- BAP:
-
benzylaminopurine
- NAA:
-
napththaleneacetic acid
- IBA:
-
indolebutyric acid
References
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Msikita, W., Skirvin, R.M. In vitro regeneration from hypocotyl and seedling cotyledons of tronchuda (Brassica oleracea var. tronchuda Bailey). Plant Cell Tiss Organ Cult 19, 159–165 (1989). https://doi.org/10.1007/BF00035815
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DOI: https://doi.org/10.1007/BF00035815