Abstract
Rapidly growing, regenerable suspension cultures were obtained from meristem-derived callus cultures of garlic (Allium sativum L.). The liquid culture medium consisted of MS salts, B5 vitamins, 3% sucrose, 1 mg l−1 naphthalene-acetic acid (NAA) and 2 mg l−1 6-benzyladenine (BA). The tissue in the suspension culture was yellow, smooth, organized, and proliferated as nodular clumps. Histological examination revealed that these morphogenic clumps had a well-defined epidermis. Following transfer of the morphogenic clumps to an agar-solidified medium, numerous meristems with green leaf primordia were produced.
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Nagasawa, A., Finer, J.J. Development of morphogenic suspension cultures of garlic (Allium sativum L.). Plant Cell Tiss Organ Cult 15, 183–187 (1988). https://doi.org/10.1007/BF00035760
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DOI: https://doi.org/10.1007/BF00035760