Abstract
Cotyledon explants of Brassica tournefortii L. were excised from germinated seedlings and cultured on Murashige & Skoog's [6] basal medium supplemented with various combinations of cytokinins and auxins, Both cytokinin and auxin were required for induction of shoot organogenesis. Of the three cytokinins tested (in combination with a low concentration of IAA), kinetin was found to be the best for shoot regeneration. On this medium, cotyledonary explants invariably underwent callusing followed by multiple shoot formation. NAA in combination with any of the three cytokinins yielded a reduced number of shoots or none, but favoured good callus growth. Callus so produced also regenerated shoots when subcultured on media containing high concentration of KIN or ZEA and low concentration of IAA. Shoots were rooted during prolonged incubation on the same medium or on MS medium free of growth regulators. Mature plants were grown in the greenhouse.
Similar content being viewed by others
References
George, L, Rao, PS (1980) In vitro regeneration of mustard plants (Brassica juncea var. Rai-5) on cotyledon explants from non-irradiated, irradiated and mutagen-treated seed. Ann Bot 46: 107–112
Jain RK, Sharma DR, Chowdhury JB. High-frequency regeneration and heritable somaclonal variation in Brassica juncea. Euphytica (in press)
Klimaszewska, K, Keller, WA (1985) High-frequency plant regeneration from thin-layer explants of Brassica napus. Plant Cell Tissue Org Cult 4: 183–197
Kumar, PR, Tsunoda, S (1980) Variation in oil content and fatty acid composition among seeds from the cruciferae. In: Tsunoda, S, Hinata, K, Gomez-Campo, C (Eds) Brassica Crops and Wild Allies, pp 235–252. Japan Scientific Societies Press, Tokyo
Mizushima, U (1980) Genome analysis in Brassica and allied genera. In: Tsunodas, Hinata, K, Gomez-Campo, C (Eds) Brassica Crops and Wild Allies, pp 89–106. Japan Scientific Societies Press, Tokyo
Murashige, T, Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco cultures. Physiol Plant 15: 473–497
Nieschlag, HJ, Wolff, IA (1971) Industrial uses of high-erucic oils. J Am Oil Chem Soc 48: 727–727
Prakash, S (1980) Cruciferous oilseeds in India. In: Tsunoda, S, Hinata, K, Gomez-Campo, C (Eds) Brassica Crops and Wild Allies, pp 151–163. Japan Scientific Societies Press, Tokyo
UN (1935) Genome analysis in Brassica with species reference to the experimental formation of B. napus and peculiar mode of fertilization. Jap J Bot 7: 389–452
Wenzel, G, Foroughi-Wehr, B, Friedt, W, Köhler, F, Oo, T (1985) Cell and tissue culture as supplementary tool in plant breeding: exemplified in potato, oilseed rape and barley. Heredias (Suppl) 3: 15–25
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jain, R.K., Chowdhury, J.B. & Friedt, W. Organogenesis and plant formation from cotyledon explant cultures of wild turnip rape (Brassica tournefortii L.). Plant Cell Tiss Organ Cult 15, 107–111 (1988). https://doi.org/10.1007/BF00035752
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00035752