Abstract
Photosynthetic CO2 assimilation, photorespiration and levels of glycollate oxidase and ribulose bisphosphate (RuBP) carboxylase were measured in barley, wheat and maize plants grown on media containing nitrate or ammonium or in plants transferred from nitrate to ammonium. The CO2 compensation point and photorespiratory CO2 release were not altered by the nitrogen growth regime nor by transfer from nitrate to ammonium. In barley and wheat plants grown on ammonium the levels of glycollate oxidase and RuBP carboxylase per unit leaf area were higher than in nitrate grown material. These differences were not evident when the results were expressed on a protein or chlorophyll basis. The ratio of glycollate oxidase activity to RuBP carboxylase activity was not altered by the nitrogen regime.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arnon DI (1949) Copper enzymes in isolated chloroplasts. Plant Physiol 24:1–15
Bird IF, Cornelius MJ and Keys AJ (1982) Affinity of RuBP carboxylases for carbon dioxide and inhibition of the enzymes by oxygen. J Exp Bot 33:1004–1013
Blackwood GC and Miflin BJ (1976) The effect of nitrate and ammonium feeding on carbon dioxide assimilation in maize. J Exp Bot 27:735–747.
Canvin DT (1979) Photorespiration: comparison between C3 and C4 plants. In Gibbs M and Latzko E, eds. Encyclopedia of Plant Physiology New Series, Vol 6, pp 368–396. Berlin: Springer Verlag
Chollet R, Ogren WL (1975) Regulation of photorespiration in C3 and C4 species. Bot Rev 41:137–179.
Emes MJ and Erismann KH (1982) The influence of the nitrogen supply on the structure and activity of glycollate oxidase in Lemna minor L. Plant Sci Letts 27:103–109
Fair P, Tew J and Cresswell CF (1974) Enzyme activities associated with carbon dioxide exchange in illuminated leaves of Hordeum vulgare L. Ann bot 38:39–43
Grossman D and Cresswell CF (1973) Influence of nitrogen supply in nutrient media on carbon dioxide compensation point (Π) of C4 photosynthetic plants. S Afr J Sci 69: 244–246
Grossman D and Creswell CF (1974) The influence of nitrate and ammonia nitrogen on the photosynthetic and photorespiratory activity of selected highveld grasses exhibiting C4 photosynthesis. Proc Grassld Soc Sth Afr 9:89–94
Hewitt EJ (1952) Sand and water culture methods used in the study of plant nutrition. Technical Communication no. 22 of the Commonwealth Bureaux of Horticulture and Plant Crops, East Malling, Kent, UK
Kanazawa T, Kirk MR and Bassham JA (1970) Regulatory effects of ammonia on carbon metabolism in photosynthezing Chlorella pyrenoidosa. Biochim Biophys Acta 205:401–408
Kerr MW and Groves D (1975) Purification and properties of glycollate oxidase from Pisum sativum leaves. Phytochem 14:359–362
Litchfield MH (1967) The automated analysis of nitrite and nitrate in blood. Analyst 92:132–136
Markwell MAK, Hass SM, Bieber LL and Tolbert NE (1978) A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem 87:206–210
Marques IA, Oberholzer MJ and Erismann KH (1983) Effects of different inorganic nitrogen sources on photosynthetic carbon metabolism in primary leaves of nonnodulated Phaseolus vulgaris L. Plant Physiol 71:555–561
McCulloch H (1967) The determination of ammonia in whole blood by a direct colorimetric method. Clin Chim Acta 17:297–304
Moss DN (1966) Respiration of leaves in light and darkness. Crop Sci 6:351–354
Peterson GL (1977) A simplification of the protein assay method of Lowry et al which is more generally applicable. Anal Biochem 83:346–356
Vaklinova S, Fedina I, Vassileva V and Ananieva L (1981) Effect of nitrate and ammonium nitrogen on the intensity of photosynthesis, photorespiration and on the ribulose-1,5-diphosphate carboxylase/oxygenase in C3 and C4 types of plants. In Akoyunoglou G ed. Photosynthesis VI. Photosynthesis and productivity, photosynthesis and environments. pp 55–62. Philadelphia: Balaban International
Willis AJ and Yemm EW (1955) The respiration of barley plants. New Phytol. 54:163–181
Yamada Y and Ikeda M (1980) In Carbon-Nitrogen Interaction in Crop Production pp 41–53. Tokyo: Japan Society for the Promotion of Science
Zelitch I (1979) Photorespiration: studies with whole tissues. In: Gibbs M and Latzko E, eds. Encyclopedia of Plant Physiology New Series, Vol 6, pp 353–367. Berlin: Springer Verlag
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hall, N.P., Reggiani, R., Franklin, J. et al. An investigation into the interaction between nitrogen nutrition, photosynthesis and photorespiration. Photosynth Res 5, 361–369 (1984). https://doi.org/10.1007/BF00034980
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00034980