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Efficiency of hydrogen photoproduction by photosystem I-enriched subchloroplast vesicles combined with Proteus mirabilis cells. Effects of some exogenous electron donors

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Abstract

High rates of hydrogen photoproduction are obtained when glutaraldehyde-fixed Photosystem I-enriched vesicles (Photosystem II-depleted) are added to hydrogenase-containing cells of Proteus mirabilis in the presence of the mediator methylviologen and a suitable electron donating system. This donor system includes ascorbate, dithioerythritol (DTE) and the mediator tetramethylphenylene-diamine (TMPD) and reduces the photosynthetic electron transfer chain at the level of plastocyanin. Both DTE and ascorbate are required for hydrogen photoproduction, DTE being the ultimate electron donor and ascorbate only having a catalytic function. Whereas the aerobic photoreduction of methylviologen is similar in the presence of DTE, ascorbate or both, under anaerobic conditions only combination of both compounds results in a high and stable amount of reduced methylviologen that can be utilized by the hydrogenase. It is concluded that oxidation reactions of reduced methylviologen, competing with the hydrogenase, rather than methylviologen photoreduction, limit hydrogen photoproduction in the presence of either DTE or ascorbate. These oxidation reactions are suggested to involve back reactions to the oxidized form(s) of ascorbate and DTE but backflow to the photosynthetic electron transfer chain (i.e. cyclic electron transfer) can not be excluded.

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Abbreviations

Tes:

N-tris (hydroxymethyl) methyl-2-aminoethanesulfonic acid

DTE:

1,4-dithioerythritol

TMPD, N,N,N′:

N′-tetramethyl-p-phenylenediamine

DCMU:

3-(3′, 4′-dichlorophenyl)-1, 1,-dimethylureum

EDAC:

1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide

DNP-INT:

2-iodo-6-isopropyl-3-methyl-2′, 4, 4′-trinitrodiphenyl ether

DBMIB:

2,5-dibromo-3-methyl-6-isopropyl-benzoquinone

PS:

photosystem

Chl:

chlorophyll

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Author notes

  1. Fons A. L. J. Peters & Ronald Boog

    Present address: Biotechnological Research, Naarden International Holland B.V., Huizerstraatweg 28, 1411 GP, Naarden, (The Netherlands)

Authors and Affiliations

  1. Biological Laboratory, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, (The Netherlands)

    Fons A. L. J. Peters, Ronald Boog, Klaas Krab & Ruud Kraayenhof

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  1. Fons A. L. J. Peters
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  2. Ronald Boog
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  3. Klaas Krab
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  4. Ruud Kraayenhof
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Peters, F.A.L.J., Boog, R., Krab, K. et al. Efficiency of hydrogen photoproduction by photosystem I-enriched subchloroplast vesicles combined with Proteus mirabilis cells. Effects of some exogenous electron donors. Photosynth Res 5, 335–346 (1984). https://doi.org/10.1007/BF00034978

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  • DOI: https://doi.org/10.1007/BF00034978

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