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On the nature of the photosynthetic energy storage monitored by photoacoustic spectroscopy

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Abstract

The photosynthetic energy storage yield of uncoupled thylakoid membranes was monitored by photoacoustic spectroscopy at various measuring beam intensities. The energy storage rate as evaluated by the half-saturation measuring beam intensity (i50) was inhibited by 3-(3,4-dichlorophenyl)-1,1 dimethylurea, by heat inactivation or by artificial electron acceptors specific for photosystem I or photosystem II; and was activated by electron donors to photosystem I. The reactions involving both photosystems were all characterized by a similar maximal energy storage yield of 16±2 percent. The data could be interpreted if we assumed that the energy storage elicited by the photosystems at 35 Hz is detected at the level of the plastoquinone pool.

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Abbreviations

PS:

photosystem

Tes:

N-Tris [hydroxymethl] methyl-2-aminoethanesulfonic acid

DCMU:

3-(3,4-dichlorophenyl)-1,1-dimethylurea

DCIP:

2,6-dichlorophenolindophenol

FeCN:

potassium ferricyanide

DCBQ:

2,5-dichlorobenzoquinone

TMPD:

N,N,N′-tetramethyl-p-phenilenediamine

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Authors and Affiliations

  1. Centre de Recherche en Photobiophysique, Université de Québec à Trois-Rivières, 3351, Boul. des Forges, C.P. 500, G9A 5H7, Trois-Rivières, Québec, Canada

    Robert Carpentier, Roger M. Leblanc & Murielle Mimeault

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  1. Robert Carpentier
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  2. Roger M. Leblanc
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  3. Murielle Mimeault
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Carpentier, R., Leblanc, R.M. & Mimeault, M. On the nature of the photosynthetic energy storage monitored by photoacoustic spectroscopy. Photosynth Res 23, 313–318 (1990). https://doi.org/10.1007/BF00034861

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  • DOI: https://doi.org/10.1007/BF00034861

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