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Mechanisms for controlling balance between light input and utilisation in the salt tolerant alga Dunaliella C9AA

Abstract

The yield of photosynthetic O2 evolution was measured in cultures of Dunaliella C9AA over a range of light intensities, and a range of low temperatures at constant light intensity. Changes in the rate of charge separation at Photosystem I (PS I) and Photosystem II (PS II) were estimated by the parameters ΦPS I and ΦPS II . ΦPS I is calculated on the basis of the proportion of centres in the correct redox state for charge separation to occur, as measured spectrophotometrically. ΦPS II is calculated using chlorophyll fluorescence to estimate the proportion of centres in the correct redox state, and also to estimate limitations in excitation delivery to reaction centres. With both increasing light intensity and decreasing temperature it was found that O2 evolution decreased more than predicted by either ΦPS I or ΦPS II. The results are interpreted as evidence of non-assimilatory electron flow; either linear whole chain, or cyclic around each photosystem.

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Abbreviations

F0 :

dark level of chlorophyll fluorescence yield (PS II centres ‘open’)

Fm :

maximum level of chlorophyll fluorescence yield (PS II centres ‘closed’)

Fv :

variable fluorescence (Fm-F0)

PS I:

Photosystem I

PS II:

Photosystem II

P700 :

reaction centre chlorophyll(s) of PS I

qN :

coefficient of non-photochemical quenching of chlorophyll fluorescence

qP :

coefficient of photochemical quenching of fluorescence yield

qE :

high-energy-state quenching coefficient

ΦPS I :

yield of PS I

ΦPS II :

yield of PS II

ΦS :

yield of photosynthetic O2 evolution

ΦP :

intrinsic yield of ‘open’ PS II centres

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Rees, D., Lee, C.B., Gilmour, D.J. et al. Mechanisms for controlling balance between light input and utilisation in the salt tolerant alga Dunaliella C9AA . Photosynth Res 32, 181–191 (1992). https://doi.org/10.1007/BF00034794

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Key words

  • chlorophyll fluorescence
  • Dunaliella
  • photosynthesis
  • quantum efficiency