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An ELISA test for BA measurement: application to wild cherry culture in vitro

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Abstract

An avidin-biotin solid phase enzyme linked immunoassay (ELISA) was developed for benzyladenine (BA) measurement in HPLC purified extracts. Its measuring range extended from 10 fmol to 10 pmol. Analysis of the immunoreactivity of all fractions obtained by HPLC fractionation of a methanolic extract from in vitro cultured wild cherry shoots showed that BA was accumulated in plantlets with 2 other immunoreactive compounds. Interassay variability never exceeded 3%.

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Authors and Affiliations

  1. Laboratoire de physiologie végétale, Université P. et M. Curie, Tour 53, 5ème étage, 4 Place Jussieu, 75252, Paris Cedex 05, France

    Philippe Label & Bruno Sotta

Authors
  1. Philippe Label
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  2. Bruno Sotta
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Label, P., Sotta, B. An ELISA test for BA measurement: application to wild cherry culture in vitro. Plant Cell Tiss Organ Cult 13, 155–158 (1988). https://doi.org/10.1007/BF00034452

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  • DOI: https://doi.org/10.1007/BF00034452

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