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Plant Cell, Tissue and Organ Culture

, Volume 38, Issue 1, pp 77–79 | Cite as

Plant regeneration from mesophyll protoplasts of Lactuca perennis

  • C. L. Webb
  • M. R. Davey
  • J. A. Lucas
  • J. B. Power
Research Note

Abstract

Cultured protoplasts of young, unexpanded leaves of the wild lettuce, Lactuca perennis, divided to produce cell colonies in an agarose-solidified, modified MS medium with reduced levels of inorganic salts, together with 2,4-d, NAA and zeatin at 0.2, 0.1 and 0.5 mg 1-1 respectively. Organogenesis followed the initial transfer of protoplastderived colonies to modified MS medium with 2,4-d, NAA and zeatin (0.1, 1.0 and 0.2 mg 1-1 respectively) and then to full-strength MS medium with 6-BA and NAA (0.4 and 0.05 mg 1-1). Shoots were rooted on agar-solidified MS medium lacking growth regulators. Regenerated shoots were established ex vitro, 21 weeks after protoplast isolation.

Key words

Lactuca perennis mesophyll protoplasts plant regeneration wild lettuce 

Abbreviations

6-BA

6-benzyladenine

BSA

bovine serum albumin

d

days

2.4-d

2,4-dichlorophenoxyacetic acid

f. wt.

fresh weight

IAA

indoleacetic acid

MES

2 [N-morpholino]ethane sulphonic acid

MS

Murashige & Skoog (1962)

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • C. L. Webb
    • 1
  • M. R. Davey
    • 1
  • J. A. Lucas
    • 1
  • J. B. Power
    • 1
  1. 1.Plant Genetic Manipulation Group, Department of Life ScienceUniversity of NottinghamNottinghamUK

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