Plant Cell, Tissue and Organ Culture

, Volume 38, Issue 1, pp 77–79 | Cite as

Plant regeneration from mesophyll protoplasts of Lactuca perennis

  • C. L. Webb
  • M. R. Davey
  • J. A. Lucas
  • J. B. Power
Research Note


Cultured protoplasts of young, unexpanded leaves of the wild lettuce, Lactuca perennis, divided to produce cell colonies in an agarose-solidified, modified MS medium with reduced levels of inorganic salts, together with 2,4-d, NAA and zeatin at 0.2, 0.1 and 0.5 mg 1-1 respectively. Organogenesis followed the initial transfer of protoplastderived colonies to modified MS medium with 2,4-d, NAA and zeatin (0.1, 1.0 and 0.2 mg 1-1 respectively) and then to full-strength MS medium with 6-BA and NAA (0.4 and 0.05 mg 1-1). Shoots were rooted on agar-solidified MS medium lacking growth regulators. Regenerated shoots were established ex vitro, 21 weeks after protoplast isolation.

Key words

Lactuca perennis mesophyll protoplasts plant regeneration wild lettuce 





bovine serum albumin




2,4-dichlorophenoxyacetic acid

f. wt.

fresh weight


indoleacetic acid


2 [N-morpholino]ethane sulphonic acid


Murashige & Skoog (1962)


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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • C. L. Webb
    • 1
  • M. R. Davey
    • 1
  • J. A. Lucas
    • 1
  • J. B. Power
    • 1
  1. 1.Plant Genetic Manipulation Group, Department of Life ScienceUniversity of NottinghamNottinghamUK

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