Abstract
The influence of temperature stress pre-treatment on anther culture response has been examined in eight commercially desirable barley cultivars. Spikes were pre-treated in darkness at 4°C for periods of 0, 7, 14, 21 and 28 days. Overall, the optimum pre-treatment period was 21 days, although there were large genotype by pre-treatment interactions. The most responsive cultivar was Igri, with a mean of 38% anthers responding, and relatively little effect of pre-treatment. The greatest effect of pre-treatment was in cv. Heriot, which had 3% response with no pre-treatment and 52% response from 14 days pre-treatment.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kasha KJ, Reinbergs E (1982) Recent developments in the production and utilization of haploids in barley. In: Proc 4th Barley Genet Symp Edinburgh pp 655–665
Kasha KJ, Kao KN (1970) High frequency haploid production in barley (Hordeum vulgare). Nature (London) 225: 874–876.
Foroughi-Wehr B, Freidt W (1984) Rapid production of recombinant barley yellow mosaic virus resistant Hordeum vulgare lines by anther culture. Theor Appl Genet 67: 377–382
Caligari PDS, Powell W, Jinks JL (1986) The use of DH for detecting linkage and pleitropy between quantitatively varying characters. J Agric Sci 106: 75–80
Powell W, Caligari PDS (1986) Investigations into the linkage of genes controlling individual quantitative characters in barley. Can J Genet Cytol 28: 63–68
Borlaug NE (1986) Plant Breeding Goals and Strategies for the 1980s. Handbook of plant cell culture, Vol. 4, ed. DA Evans, WR Sharp, PV Ammirato, pp. 3–12
Dunwell JM (1985) Anther and ovary culture. In: Bright SWJ, Jones MGK (eds) Cereal and tissue cell culture. Nijhoff/Junk, Dordrecht pp. 1–44
Haung B, Sunderland N (1982) Temperature-stress pre-treatment in barley anther culture. Ann Bot 49: 77–78
Marsolais AA, Kasha KJ (1985) Callus induction from barley microspores. The role of sucrose and auxin in a barley anther culture medium. Can J Bot 63: 2209–2212
Lyne LR, Bennet RI, Hunter CP (1986) Embryoid and plant production from cultured barley anthers. In Anderson PG, Withers LA (eds) Plant Tissue Culture and its Agriculture Applications, Butterworths, Guildford pp. 405–411
Foroughi-Wehr B, Mix C, Call H, Wilson HM (1976) Plant production from cultured anthers of Hordeum vulgare L. Z Planzenzllcht 77: 198–204
Datta SK (1987) Plant regeneration by pollen embryogenises from cultured whole spikes of barley (Hordeum vulgare). Theor. Appl. Genet 74: 121–124
Wilson HM (1977) Culture of whole barley spikes stimulates high frequencies of pollen calluses in individual anthers. Plant Sci Letts 9: 233–239
Sunderland N, Huang B, Hills GJ (1984) Disposition of pollen in situ and its relevance to anther/pollen culture. J Exp Bot 85: 521–530
Dunwell JM, Francis RJ, Powell W (1987) Anther culture of Hordeum vulgare L: A genetic study of microspore callus production and differentiation. Theor Appl Genet (In press)
Hunter CP (1985) The effect of anther orientation on the production of microsporederived embryoids and plants of Hordeum vulgare cv. Sabarlis. Plant Cell Rep 4: 267–268
Powell W, Borrino EM, Goodall V (1987) The effect of anther orientation on microsporederived plant production in barley (Hordeum vulgare L.). Euphytica (In press)
Shannon PRM, Nicholson AE, Dunwell JM and Davies DR (1985) Effect of anther orientation on microspore-callus production in barley (Hordeum vulgare L.). Plant Cell Tissue Organ Cult 4: 271–280
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Powell, W. The influence of genotype and temperature pre-treatment on anther culture response in barley (Hordeum vulgare L.). Plant Cell Tiss Organ Cult 12, 291–297 (1988). https://doi.org/10.1007/BF00034371
Issue Date:
DOI: https://doi.org/10.1007/BF00034371