Abstract
A method is described for regenerating callus from mesophyll protoplasts of a winter variety of Brassica napus. The method combines the use of Ficoll in an initial liquid medium, enhancing early protoplast division and cell colony formation, with a transfer to an agarose system after 10 days culture to give rapid microcalli formation. Further transfers resulted in callus regeneration and the initiation of organogenesis.
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Millam, S.R., Burns, A.T.H., Hocking, T.J. et al. Effects of a Ficoll-agarose system on early division and development of mesophyll protoplasts of winter oilseed rape (Brassica napus L.). Plant Cell Tiss Organ Cult 12, 285–290 (1988). https://doi.org/10.1007/BF00034370
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DOI: https://doi.org/10.1007/BF00034370