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The production of strawberry plants from callus cultures

Plant Cell, Tissue and Organ Culture volume 12, pages 235–241 (1988)Cite this article

Abstract

Shoots were regenerated from callus of the commercially important strawberry varieties Bogota, Brighton, Cambridge Favourite, Hapil, Ostara, Rapella, Red Gauntlet and JILA33 which is a promising selection from a current breeding programme.

The callus was initiated from explants of petiole or lamina of leaves of micropropagated shoots in vitro or of lamina or peduncle from greenhouse plants. There was more shoot regeneration with callus from lamina than from petiole although with the variety Hapil, regeneration occurred only with callus from peduncle.

With seven of the varieties, shoot regeneration occurred on culture media with BAP and 2,4-D whilst with the remaining variety, Cambridge Favourite, it occurred only with medium which contained 1AA-β alanine conjugate in place of 2,4-D.

Regenerated shoots rooted readily and the plants produced are being studied for somaclonal variation.

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References

  1. Anderson HM, Abbott AJ, Wiltshire S (1982) Micropropagation of strawberry plants in vitro — effect of growth regulators on the incidence of multi-apex abnormality. Scientia Hort 16: 331–341

    Google Scholar 

  2. Good NE, Hangarter R, Carlson P (1982) The use of conjugates of indoleacetic acid as auxins in tissue culture. In: Earle ED, Demarly Y (eds) Variability in Plants Regenerated from Tissue Culture. New York: Praeger pp 140–147

    Google Scholar 

  3. John A (1986) Vitrification in Sitka Spruce Cultures. In: Withers LA, and Alderson PG (eds) Plant Tissue Culture and its Agricultural Applications. Butterworths pp 167–174

  4. Scowcroft WR, Larkin PJ (1982) Somaclonal variation: a new option for plant improvement. In: Vasil IK, Scowcroft WR. Frey KJ (eds) Plant Improvement and Somatic Cell Genetics. Academic Press Inc pp 159–178

  5. Lee YB, Kim YR (1979) Shoot formation in tissue culture of petiole and leaf blade segments of strawberry. Studies from the Institute of Horticulture, Kyoto University 9: 78–82

    Google Scholar 

  6. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15: 473–497

    Google Scholar 

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Authors and Affiliations

  1. Institute of Horticultural Research, East Malling, ME19 6BJ, Maidstone, Kent, UK

    O. P. Jones, Barbara J. Waller & M. G. Beech

Authors
  1. O. P. Jones
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  2. Barbara J. Waller
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  3. M. G. Beech
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Jones, O.P., Waller, B.J. & Beech, M.G. The production of strawberry plants from callus cultures. Plant Cell Tiss Organ Cult 12, 235–241 (1988). https://doi.org/10.1007/BF00034364

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  • DOI: https://doi.org/10.1007/BF00034364

Key words

  • tissue culture
  • callus
  • organogenesis
  • strawberry
  • somacloning