Abstract
Embryogenic culture was induced from the immature embryos of Quercus serrata using Marashige and Skoog's medium (MS) containing 0.1 μM each of 2,4-d and BAP, and subcultured for seven months before isolation of protoplasts by using 1% Cellulase RS in 0.6 M mannitol solution. Efficient colony formation was obtained when protoplasts were cultured in a liquid MS medium containing 0.6 M mannitol, 3% sucrose and combination of 0.1 μM or 1 μM each of 2,4-d and BAP. Excluding ammonium nitrate from the MS medium resulted in the decrease of the percentage of colony formation. From colonies, both agar culture and liquid culture were sustained in the MS media without mannitol containing no plant growth regulator, or containing 0.1 μM of BAP in combination with 0.1 μM or 1 μM of 2,4-d.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BAP:
-
6-benzylaminopurine
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- MS:
-
medium after Murashige & Skoog (1962).
References
Attree SM, Dunstan DI & Fowke LC (1989) Initiation of embryogenic callus and suspension cultures, and improved embryo regeneration from protoplasts, of white spruce (Picea glauca). Can. J. Bot. 67: 1790–1795
Fry SC (1990) Roles of the primary cell wall in morphogenesis. In: Nijkamp HJJ et al. (Eds) Progress in Plant Cellular and Molecular Biology (pp 504–513). Kluwer Academic Publishers, Dordrecht
Klimaszewska K (1989) Recovery of somatic embryos and plantlets from protoplast cultures of Larix × eurolepis. Plant Cell Rep. 8: 440–444
Koyama M, Hosoi Y & Saito A (1988) Isolation, culture and division of protoplasts from Konara (Quercus serrata) callus cultures. J. Jpn For. Sci. 70: 231–233
Lang H & Kohlenbach HW (1989) Cell differentiation in protoplast cultures from embryogenic callus of Abies alba L. Plant Cell Rep. 8: 120–123
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Sasamoto H, Hosoi Y, Ishii K, Sato T & Saito A (1989a) Factors affecting the formation of callus from leaf protoplasts of Populus alba. J. Jpn For. Soc. 71: 449–455
Sasamoto H, Hosoi Y, Ishii K, Sato T & Saito A (1989b) Organs differentiation from the protoplasts-derived calli and from the suspension cultures of Populus alba leaves. Trans. 100th Mtg. Jpn For. Soc. 100: 529–530
Sasamoto H, Hosoi Y, Ishii K, Sato T & Saito A (1989c) Callus formation and plantlet regeneration from mesophyll protoplasts of Populus sieboldii. Trans. 100th Mtg. Jpn For. Soc. 100: 531–532
Sasamoto H & Hosoi Y (1989) Somatic embryogenesis in suspension cultures of Quercus serrata Thunb. J. Jpn For. Soc. 71: 20–22
Sasamoto H & Hosoi Y (1990) Effects of electric fusion on the calli formation and regeneration from the protoplasts of Quercus and two Populus species. In: Abstracts VIIth Int. Cong Plant Tissue and Cell Culture (p. 36)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sasamoto, H., Hosoi, Y. Callus proliferation from the protoplasts of embryogenic cells of Quercus serrata . Plant Cell Tiss Organ Cult 29, 241–245 (1992). https://doi.org/10.1007/BF00034359
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00034359