Summary
The acid phosphatase isoenzyme patterns of extracts of seeds from Brassica oleracea subspecies and Brussels sprouts cultivars have been analysed. In view of the inhomogeneity of most seed material, the extracts must be prepared from single seeds if the patterns are to be used for taxonomic classification.
The acid phosphatase isoenzymes located in the 15–20 mm region of the electropherograms proved to be controlled by one locus, which consists of 4 alleles acting without dominance. As a result, the acid phosphatase patterns of seeds of pure inbred lines are characterized by a single band in this region. Crossing of inbred lines with a different allele constitution gives hybrid seed with the related double-band pattern. No polypeptide hybridization occurs.
By analysis of the acid phosphatase patterns of sufficient individual seeds in a seed sample from a new Brussels sprouts hybrid, it was possible to determine the proportion of inbred seed in the sample. The results were in good agreement with those obtained from field observations.
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Nijenhuis, B.T. Estimation of the proportion of inbred seed in Brussels sprouts hybrid seed by acid phosphate isoenzyme analysis. Euphytica 20, 498–507 (1971). https://doi.org/10.1007/BF00034204
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DOI: https://doi.org/10.1007/BF00034204