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The types of bioreactors used for shoots and embryos

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Abstract

Plant regenerated organs such as shoots, bulbs, microtubers, corms, embryos, etc. have been successfully proliferated in the bioreactor. The use of a bioreactor leads to the development of technology suitable for large scale plant propagation. The basic construction and characteristics of various types of bioreactor systems are reviewed in relation to shoot and embryo cultures. A pilot scale 500 liter bioreactor system was applied to the production of large scale Stevia rebaudiana shoots.

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Abbreviations

DW:

dry weight

EC:

electrical conductivity

FW:

fresh weight

ORP:

oxidation-reduction potential

References

  • Akita M & Takayama S (1994a) Effects of medium surface level control on the mass propagation of potato tubers using a jar fermentor culture technique. Plant Tiss. Cult. Lett. 10: 242–248

    Google Scholar 

  • Akita M & Takayama S (1994b) Stimulation of potato (Solanum tuberosum L.) tuberization by semicontinuous liquid medium surface level control. Plant Cell Rep. 13: 184–187

    Google Scholar 

  • Akita M, Shigeoka T, Koizumi Y & Kawamura M (1994) Mass propagation of shoots of Stevia rebaudiana using a large scale bioreactor. Plant Cell Rep. 13: 180–183

    Google Scholar 

  • Cazzulino DL, Pedersen H, Chin CK & Styer D (1990) Kinetic of carrot somatic embryo development in suspension culture. Biotechnol. Bioeng. 35: 781–786

    Google Scholar 

  • Cazzulino D, Pedersen H & Chin CK (1991) Bioreactors and image analysis for scale-up and plant propagation. In: Vasil I K (Ed) Cell Culture and Somatic Cell Genetics of Plants Vol 8, Scale-Up and Automation in Plant Propagation (pp 147–177). Academic Press, San Diego

    Google Scholar 

  • Denchev PD, Kuklin AI & Scragg AH (1992) Somatic embryo production in bioreactors. J. Biotechnol. 26: 99–109

    Google Scholar 

  • Geier T, Beck A & Preil W (1992) High uniformity of plants regenerated from cytogenetically variable embryogenic suspension cultures of poinsettia Euphorbia pulcherrima Willd. Plant Cell Rep. 11: 150–154

    Google Scholar 

  • Greidziak N, Diettrich B & Luckner M (1990) Batch cultures of somatic embryos of Digitalis lanata in gaslift fermenters development and cardenolide accumulation. Planta Med. 56: 175–178

    Google Scholar 

  • Gupta PK, Timmis R, Pullman G, Yancey M, Carlson W & Carpenter C (1991) Development of an embryogenic system for automated propagation of forest trees, In: Vasil I K (Ed) Cell Culture and Somatic Cell Genetics of Plants Vol 8, Scale-Up and Automation in Plant Propagation (pp 75–93). Academic Press, San Diego

    Google Scholar 

  • Huang LC, Vits H, Staba EJ, Cooke TJ & Hu HS (1992) Effect of cultivation age and embryo size on specific oxygen uptake rate in developing somatic embrhyos of Daucus carota L. Bio-technol. Let. 14: 701–706

    Google Scholar 

  • Ikeda H (1985) Culture vessel for photoautotrophic culture. Japanese Patent, Kokai. 60–237984 (in Japanese)

  • Inoue M (1984) Culture instrument for photoautotrophic orgaisms. Japanese Patent, Kokai. 59–21682 (in Japanese)

  • Ishibashi T, Odawara Y, Fujiwara K & Ookuma M (1987) New oxygen supplying method for animal cell reactors, Hitachi Hyouron 69: 301–305 (in Japanese)

    Google Scholar 

  • Jay V, Genestier S & Courduroux JC (1992) Bioreactor studies on the effect of dissolved oxygen concentrations on growth and differentiation of carrot (Daucus carota L.) cell cultures. Plant Cell Rep. 11: 605–608

    Google Scholar 

  • Kessel RHJ & Carr AH (1972) The effect of dissolved oxygen concentration on growth and differentiation of carrot (Daucus carota) tissue. J. Exp. Bot. 23: 996–1007

    Google Scholar 

  • Katagi H, Takahasi E, Nakao K & Inui M (1986) Shoot-forming culture of Pelargonium graveolens by jar fermentation. Nippon Nogeikagaku Kaishi 6: 15–17 (In Japanese)

    Google Scholar 

  • Kitto SL & Janick J (1985a) Production of synthetic seeds by encapsulating asexual embryos of carrot. J. Amer. Soc. Hozt. Sci. 11: 277–282

    Google Scholar 

  • Kitto SL & Janick J (1985b) Hardening treatments increase survival of synthetically-coated asexual embryos of carrot. J. Amer. Soc. Hozt. Sci. 110: 283–286

    Google Scholar 

  • Kitto SL & Janick J (1985c) A citrus embryo assay to screen water-soluble resins as synthetic seed coats. HortSci. 20: 98–100

    Google Scholar 

  • Kiyosue T, Kamada H & Harada H (1989) Induction of somatic embryogenesis from carrot seeds by hypocotyl treatment., Plant Tiss. Cult. Lett. 6: 138–143

    Google Scholar 

  • Leifert C & Waites WM (1990) Contaminants of plant tissue cultures., Newsletter, IAPTC. No. 60: 1–13

  • Lulsdorf MM, Tautorus TE, Kikcio SI, Bethune TD & Dunstan DI (1993) Germination of encapsulated embryos of interior spruce (Picea glauca engelmannii comlex) and black spruce (Picea mariana Mill). Plant Cell Rep. 12: 385–389

    Google Scholar 

  • Manfredini R, Saporiti LG & Cavallera V (1982) Technological approach to industrial fermentation: limiting factors and practical solutions. La Chimica E'Industria 4: 323–334

    Google Scholar 

  • Nursery Technology Inc. (1993) Development of the technology for ruce seedling production system using biorechnology, In: Institute Report (pp II-111–123). Nursery Technology Inc., Tokyo (in Japanese)

    Google Scholar 

  • Phillips G & Collins GB (1981) Induction of development of somatic embryos from cell suspension cultures of soybean, Plant Cell Tiss. Org. Cult. 1: 123–129

    Google Scholar 

  • Preil W, Florek P, Wix U & Beck A (1988) Towards mass propagation by use of bioreactors, Acta Hortic. 226: 99–10

    Google Scholar 

  • Preil W (1991) Application of bioreactor in plant propagation. In: Debergh PC & Zimmermann RH (Eds) Micropropagation: Technology and Application (pp 425–446). Kluwer Academic Publishers Group, Dordrecht, Netherlands

    Google Scholar 

  • Redenbaugh K, Viss P, Slade D & Fujii JA (1987) Scale-up: artificial seeds, In: Plant tissue and Cell Culture (pp 473–493). Alan R. Liss, Inc

  • Redenbaugh K, Fujii JA & Slade D (1991a) Synthetic seed technology, In: Vasil I K (Ed) Cell Culture and Somatic Cell Genetics of Plants, Vol 8, Scale-Up and Automation in Plant Propagation (pp 36–74). Academic Press, San Diego

    Google Scholar 

  • Redenbaugh K, Fujii J, Slade D, Viss P & Kossler M (1991b) Artificial seeds-Encapsulated somatic embryos, In: Bajaj YPS (Ed) Biotechnology in Agriculture and Forestry, Vol 17 (pp 395–416). Springer Verlag, Berlin

    Google Scholar 

  • Sharma NK (1992) Automation in plant tissue culture: problems and prospects. Curr. Sci. 62: 507–512

    Google Scholar 

  • Stuart DA, Strickland SG & Walker KA (1987) Bioreactor production of alfalfa somatic embryos, HortSci. 22: 800–803

    Google Scholar 

  • Styer DJ (1985) Bioreactor technology for plant propagation. In: Henke R R & Hughes K W (Eds) Tissue Culture in Forestry and Agriculture (pp 117–130). Plenum Press, New York

    Google Scholar 

  • Takayama S & Misawa M (1981) Mass propagation of Begonia x hiemalis plantlets by shake culture. Plant Cell Physiol. 22: 461–467

    Google Scholar 

  • Takayama S, Arima Y & Akita M (1986) Mass propagation of plants by fermentor culture techniques. Abst. 6th International Congress of Plant Tissue and Cell Culture (pp 449). Internationl Association of Plant Tissue Culture, University of Minnesota

  • Takayama S (In press) Bioreactors for plant cell tissue and organ cultures, In: Vogel H C (Ed) Fermentation and Biotechnology Handbook 2nd ed. Noyes Publishing Co., Ltd, San Diego

  • Takayama S (1991) Mass propgation of plants through shake- and bioreactor-culture techniques. Biotechnology in Agriculture and Forestry, Vol 17 (pp 495–515). Springer Verlag, Berlin

    Google Scholar 

  • Takayama S, Swedlund B & Miwa Y (1991a) Automated propagation of microbulbs of lilies. In: Vasil IK (Ed) Cell Culture and Somatic Cell Genetics of Plants, Vol 8 (pp 111–131). Academic Press, Inc

  • Takayama S, Amo T & Fukano M (1991b) Rapid clonal propagation of Hyacinthus orientalis bulbs by shake culture, Scientia Hortic. 45: 315–321

    Google Scholar 

  • Takayama S, Miwa Y, Imaizumi K & Aiga I (1990) Measurement of cell mass in plant cell and tissue cultures by transmitted light and reflected light. Abst. 1990 Meeting of Jap. Soc. High Technol. Agric. (pp 63–64) (in Japanese)

  • Takayama S & Tsumaki N (1992) Measurement of cell mass by exponential decay constant by electric discharge. Abst. 1992 Meeting of Jap. Soc. High Technol. Agric. (pp 69–70) (in Japanese)

  • Tanaka H, Nishijima F, Suwa M & Iwamoto T (1983) Rotating drum fermentor for plant cell suspension cultures, Biotechnol. Bioeng. 25: 2359–2370

    Google Scholar 

  • Tautorus TE, Lulsdorf MM, Kikcio SI & Dunstan DI (1992) Bioreactor culture of Picea mariana Mill. black spruce and the species complex Picea glauca Engelmann. II. Interior spruce somatic embryo growth parameters. Appl. Microbiol. Biorechnol. 38: 46–51

    Google Scholar 

  • Teng WL, Lin CP & Liu AJ (1993) Regenerating lettuce from suspension culture in a 2-liter bioreactor. HortSci. 28: 669–671

    Google Scholar 

  • Terashima T & Nishimura S (1991) Mass propagation of somatic embryos in carrot (Daucus carota) using bioreactor. The effects of stirring methods and dissolved oxygen concentration on the embryogenesis. Ikusyugaku Zasshi, 41 (Suppl. 2): 234–235 (in Japanese)

    Google Scholar 

  • Torii T, Kan M, Okamoto T, Kitani T & Takayama S (1992) Measurement of plant tissue cultures by ultrasonic waves. Abst. 1992 Meeting of Jap. Soc. High Technol. Agric. (pp 67–68) (in Japanese)

  • Ushiyama K (1988) Large scale culture techniques of plant cells and the secondary metabolite production. Hakko to Kogyo 46: 7–11 (in Japanese)

    Google Scholar 

  • Ushiyama K, Miyamoto Y, Oda H & Ishida Y (1984a) Gaseous phase culture equipment for continuous culture of plant tissues, Japanese Patent, Kokai. 59–45873 (in Japanese)

  • Ushiyama K, Oda H, Miyamoto Y & Ishida Y (1984b) Gaseous phase culture method and the equipment for plant tissues, Japanese Patent, Kokai. 59–45879 (in Japanese)

  • Walker KA & Sato SJ (1981) Morphogenesis in callus tissue of Medicago sativa: the role of ammonium ion in somatic embryogenesis. Plant Cell Tiss. Org Cult. 1: 109–121

    Google Scholar 

  • Wheat D, Bondryk RP & Nystrom J (1986) Spin filter bioreactor technology as applied to industrial plant propagation. HortSci. 21: 819

    Google Scholar 

  • Ziv M & Ariel T (1991) Bud proliferation and plant regeneration in liquid-cultured Philodendron treated with ancymidol and paclbutrazol. J. Plant Growth Regul. 10: 53–57

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Biological Science and Technology, Tokai University, 317 Nishino, 410-03, Numazu, Shizuoka, Japan

    Shinsaku Takayama

  2. Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Uchita, Naga, 649-64, Wakayama, Japan

    Motomu Akita

Authors
  1. Shinsaku Takayama
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  2. Motomu Akita
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Takayama, S., Akita, M. The types of bioreactors used for shoots and embryos. Plant Cell Tiss Organ Cult 39, 147–156 (1994). https://doi.org/10.1007/BF00033922

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