Effects of auxins and cytokinins on epigenetic instability of callus-propagated Kalanchoe blossfeldiana Poelln.
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A main problem in the vegetative propagation of ornamental plants in vitro is the epigenetic instability of cells removed from their organized environment. With calluses of leaf explants of Kalanchoe blossfeldiana Poelln., cv. Yucatan, the role of plant growth regulators (PGRs) in the occurrence of fasciation was studied.
In various combinations of auxins and cytokinins, the auxin 2,4-D (2,4-dichlorophenoxyacetic acid) gave only deformed, inseparable shoot primordia. The most rapid callus induction with regeneration of well-developed sprouts was obtained with the natural IAA (indoleacetic acid) and Z (zeatin).
As a first symptom of fasciation, aberration in decussate phyllotaxis can be observed. At increasing concentrations of IAA + Z, this symptom gradually decreased but fasciation proper increased. The optimum concentration was at 1 μM for both PGRs. Reduction of exposure to the PGRs from six to three weeks reduced the epigenetic instability.
Key wordsKalanchoe in vitro propagation callus epigenetic instability fasciation plant growth regulators (PGRs)
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- 1.Cassells AC (1985) Genetic, epigenetic and non-genetic variation in tissue culture derived plants. In: Schafter-Menuhr A (Ed) In vitro techniques-propagation and long term storage (pp 111–120). Martinus Nihjhoff/Dr W. Junk, DordrechtGoogle Scholar
- 2.Christianson ML, Warnick DA (1983) Competence and determination in the process of in vitro shoot organogenesis. Developm Biol 95: 288–293Google Scholar
- 3.Christianson ML, Warnick DA (1984) Phenocritical times in the process of in vitro shoot organogenesis. Developm Biol 101: 382–390; Biol Plant 14: 177–185Google Scholar
- 4.Dunlap JR, Kresovich S, McGee RE (1986) The effect of salt concentration on auxin stability in culture media. Plant Physiol 81: 934–936Google Scholar
- 5.Everett N (1982) The determination phase of differentiation. Proc 5th Int Congr Plant Tiss Cell Cult (pp 93–94)Google Scholar
- 6.Gorter ChJ (1965) Origin of fasciation. In: Ruhland W (Ed) Encyclopedia of Plant Physiology XV/2 (pp 330–351). Springer-Verlag, BerlinGoogle Scholar
- 7.Huxter TJ, Thorpe TA, Reid DM (1976) Shoot initiation in light- and dark-grown tobacco callus: the role of ethylene. Physiol Plant 53: 319–326Google Scholar
- 8.Kevers C, Gaspar Th (1986) Vitrification of carnation in vitro: changes in water content, extracellular space, air volume, and ion levels. Physiol Veg 24: 647–653Google Scholar
- 9.Meins FJr (1983) Heritable variation in plant cell culture. Ann Rev Plant Physiol 34: 327–346Google Scholar
- 10.Meins FJr (1986) Phenotypic stability and variation in plants. Curr Topics Developm Biol 20: 373–381Google Scholar
- 11.Meins FJr, Foster R (1986) Transdetermination of plant cells. Differentiation 30: 188–189Google Scholar
- 12.Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473–497Google Scholar
- 13.Schneider-Moldrickx R (1983) The influence of light quality and light intensity on regeneration of Kalanchoe-blossfeldiana-hybrids in vitro. Acta Hort 131: 163–169Google Scholar
- 14.Schneider-Moldrickx R, Horn W (1984) Vermehrung von Kalanchoe-blossfeldiana-Hybriden in vitro. I. Die Sprossbildung an Blattspreiten-explantaten. Gartenbauwissenschaft 49: 230–234Google Scholar
- 15.Smith RH, Nightingale AE (1979) In vitro propagation of Kalanchoe. HortScience 14: 20Google Scholar
- 16.Theiler-Hedtrich R, Koch C, Meier F (1983) ‘In vitro’-Kultur von Kalanchoe blossfeldiana-Hybriden. Der Gartenbau 104: 679–683Google Scholar
- 17.Thimann KV, Sachs T (1966) The role of cytokinins in the ‘fasciation’ disease caused by Cornebacterium fascians. Am J Bot 53: 731–739Google Scholar
- 18.White OE (1948) Fasciation. Bot Rev 14: 319–351Google Scholar