Abstract
Shoot tips from seedlings of Digitalis thapsi L. were cultured on Murashige and Skoog's medium and the effect of various auxins (2,4-D, NAA and IAA) were analyzed alone or in combination with cytokinis (BA and kinetin). Shoot multiplication and direct rooting of the new shoots were obtained after four weeks of culture in MS medium without hormones, but callus formation and the appearance of abnormal phenotypes were frequent. The addition of auxins to the cultures prevented the formation of callus but not the appearance of variant phenotypes. Both drawbacks could be avoided by combination of NAA or IAA with BA or kinetin. The best results for shoot multiplication and direct rooting were obtained with 0.5 mg l-1 NAA and 0.1 or 0.5 mg l-1 kinetin.
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Abbreviations
- BA:
-
6-benciladenine
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IAA:
-
indole-3-acetic acid
- Kin:
-
kinetin
- NAA:
-
naphtalene acetic acid
- MS:
-
Murashige and Skoog
References
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Herrera, M.T., Cacho, M., Corchete, M.P. et al. One step shoot tip multiplication and rooting of Digitalis thapsi L.. Plant Cell Tiss Organ Cult 22, 179–182 (1990). https://doi.org/10.1007/BF00033633
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DOI: https://doi.org/10.1007/BF00033633