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Testing of different antibiotics against Gram-positive and Gram-negative bacteria isolated from plant tissue culture

Plant Cell, Tissue and Organ Culture volume 29, pages 139–144 (1992)Cite this article

Abstract

Different Gram-positive and Gram-negative bacteria (Staphylococcus xylosus, S. aureus, S. cohnii, Bacillus sp., Corynebacterium sp., Pseudomonas vesicularis) were isolated from homogenized shoot tips of Drosera rotundifolia, Spatiphyllum sp., Syngonium cv. White butterfly, Nephrolepis exaltata cv. Teddy Junior. Growth inhibition of selected bacterial strains was examined using 28 different single antibiotics and 7 antibiotic mixtures. It was found that with the two mixtures Imipenem/Ampicillin and Imipenem/Penicillin G at concentrations of 5 mg l−1 each, bacterial growth inhibition was most effective. Because of the lack of toxic effects on in vitro plants of 7 species it was proposed that these antibiotic mixtures can be applied advantageously to inhibit bacterial growth in tissue culture.

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References

  • Attafuah A & Bradbury JF (1989) Pseudomonas antimicrobica, a new species strongly antagonistic to plant pathogens. J. Appl. Bact. 67: 567–573

    Google Scholar 

  • Boxus PH & Terzi J-M (1987) Big losses due to bacterial contamination can be avoided in mass propagation scheme. Acta Hort. 212: 91–93

    Google Scholar 

  • Cornu D & Michel MF (1987) Bacterial contaminants in shoot cultures of Prunus avium L., choice and phytotoxicity of antibiotics. Acta Hort. 212: 83–86

    Google Scholar 

  • George EF & Sherrington PD (1984) Plant Propagation by Tissue Culture (pp 88–93). Exegetics Ltd, England

    Google Scholar 

  • Holt JG (1986) Bergey's Manual of Systematic Bacteriology, Vol 2. Williams & Wilkins, Baltimore, London, Los Angeles, Sydney

    Google Scholar 

  • Leifert C, Waites WM & Nicholas JR (1989) Bacterial contaminants in micropropagated plant cultures. J. Appl. Bact. 67: 353–361

    Google Scholar 

  • Leifert C, Waites WM, Nicholas JR & Keetley JWW (1990) Yeast contaminants of micropropagated plant cultures. J. Appl. Bact. 69: 471–476

    Google Scholar 

  • Lloyd G & McCown B (1981) Commercially-feasible micropropagation of Mountain Laurel, Kalmia latifolia, by use of shoot tip culture. Int. Plant Prop. Soc. Proc. 30: 421–427

    Google Scholar 

  • Mathias PJ, Alderson PG & Leaky RRB (1987) Bacterial contamination in hardwood cultures. Acta Hort. 212: 43–48

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Okkels FT & Pederson MG (1983) The toxicity to plant tissue and to Agrobacterium tumefaciens of some antibiotics. Acta Hort. 225: 199–207

    Google Scholar 

  • Podwyszynska M & Hempel M (1987) Identification and elimination of ‘slowly growing’ bacteria from micropropagated Gerbera. Acta Hort. 212: 112

    Google Scholar 

  • Trick I & Lingens F (1985) Aerobic spore-forming bacteria as detrimental infectants in plant tissue cultures. Appl. Microbiol. Biotechnol. 21: 245–249

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Dairy Research and Bacteriology, Agricultural University, Gregor Mendel-Str. 33, A-1180, Vienna, Austria

    W. Kneifel

  2. Department of Botany, Agricultural University, Gregor Mendel-Str. 33, A-1180, Vienna, Austria

    W. Leonhardt

Authors
  1. W. Kneifel
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  2. W. Leonhardt
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Kneifel, W., Leonhardt, W. Testing of different antibiotics against Gram-positive and Gram-negative bacteria isolated from plant tissue culture. Plant Cell Tiss Organ Cult 29, 139–144 (1992). https://doi.org/10.1007/BF00033619

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  • DOI: https://doi.org/10.1007/BF00033619

Key words

  • antibiotics
  • bacterial identification
  • growth inhibition
  • plant tissue culture