Abstract
Three commercial varieties of flue-cured tobacco, Chatham, Delcrest and Hicks, were subjected to irradiation by thermal neutrons at six doses. Germination of the treated seeds was good in the lowest two doses, 1×1012 and 2.5×1012 N/cm2/sec. while deterioration started in 5×1012 which was considered to be the threshold dose. The other three doses, 1×1013, 2.5×1013 and 5×1013 proved to be lethal.
The field population of about 1,200 N1 plants was screened for pollen fertility. About 22 per cent. of the plants showed less than 80 per cent. pollen fertility and in two flower mutants there was complete absence of pollen.
Chlorophyll mutants in the N2 progeny consisted of albinas which appeared as simple recessives in a monohybrid ratio.
Morphological leaf mutants, mostly due to chromosomal aberrations such as single or double or reciprocal translocation, fragmentation, etc. were isolated under different leaf types. Some new leaf mutants breeding true were isolated and maintained for field performance.
Flower mutants such as male sterile, yellow stigma and split flower were isolated in the N1, N2 and N3 progenies.
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Murty, G.S., Krishnamurty, K.V. & Rao, K.A. Cytogenetics of neutronic mutants in Nicotiana tabacum . Euphytica 12, 57–68 (1963). https://doi.org/10.1007/BF00033594
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DOI: https://doi.org/10.1007/BF00033594