Abstract
Leaf segments of a monohaploid, dihaploid and tetraploid genotype of the potato (Solanum tuberosum; x = 12) were cultured on callus-inducing medium with 10, 20, 30 or 40 gl−1 sucrose. After 5 and 7 days of culture, metaphases contained the somatic or polyploidized number of mono- or diplochromosomes. The percentages of polyploidized metaphases were inversely correlated with the number of chromosome sets of the genotypes. In monohaploid leaf segments the percentages of polyploidized metaphases and of metaphases with diplochromosomes increased when the sucrose concentration was raised from 10 or 20 to 30 gl−1 and remained constant or decreased from 30 to 40 gl−1. Higher concentrations of sucrose but not higher osmolalities of the medium due to mannitol induced endoreduplication in more cells. The frequency of polyploidized metaphases and metaphases with diplochromosomes in dihaploid and tetraploid leaf segments remained constant through increases in sucrose concentrations.
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References
Skirvin RM (1978) Natural and induced variation in tissue culture. Euphytica 27: 241–266
Bayliss MW (1980) Chromosomal variation in plant tissues in culture. Int Rev Cytol Suppl 11A: 113–144
Constantin MJ (1981) Chromosome instability in cell and tissue cultures and regenerated plants. Environ Exp Bot 21: 359–368
Gould AR (1983) Control of the cell cycle in cultured cells. CRC Crit Rev Plant Sci 1: 315–344
D'Amoto F (1985) Cytogenetics of plant cell and tissue cultures and their regenerates. CRC Crit Rev Plant Sci 3: 73–112
Kovács EI (1985) Regulation of karyotype stability in tobacco tissue cultures of normal and tumorous genotypes. Theor Appl Genet 70: 548–554
Kar DK, Sen S (1985) Effect of hormone on chromosome behaviour in callus cultures of Asparagus racemosus. Biol Plant 27: 6–9
Sengupta J, Sen S (1987) Comparative analysis of the effect of growth hormone on morphology and cytology of callus culture of Crepis tectorum L. Caryologia 40: 221–227
Zhang DL, Li KQ, Gu W, Hao LF (1987) Chromosome aberration and ploidy equilibrium of Vicia faba in tissue culture. Theor Appl Genet 75: 132–137
Cambecedes J, De Boucaud M-T, Gaultier J-M, Lutz A (1988) Nuclear behaviour of colonies and regenerated buds from protoplasts of Nicotiana plumbaginifolia Viviani haploids. Plant Cell Tissue Organ Culture 13: 27–37
Pijnacker LP, Walch K, Ferwerda MA (1986) Behaviour of chromosomes in potato leaf tissue cultured in vitro as studied by BrdC-Giemsa labelling. Theor Appl Genet 72: 833–839
Pijnacker LP, Sree Ramulu K, Dijkhuis P, Ferwerda MA (1989) Flow cytometric and karyological analysis of polysomaty and polyploidization during callus formation from leaf segments of various potato genotypes. Theor Appl Genet 77: 102–110
Bokelmann GS, Roest S (1983) Plant regeneration from protoplasts of potato (Solanum tuberosum cv. Bintje). Z Planzenphysiol 109: 259–265
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Pijnacker LP, Ferwerda MA (1984) Giemsa C-banding of potato chromosomes. Can J Genet Cytol 26: 415–419
Nagl W (1978) Endopolyploidy and Polyteny in Differentiation and Evolution (283 pp) Elsevier North-Holland, Amsterdam/New York/Oxford
Maretzki A, Thorn M, Nickell LG (1974) Utilization and metabolism of carbohydrates in cell and callus cultures. In: Street HE (Ed) Tissue Culture and Plant Science (pp 329–361) Academic Press, London/New York
Fowler MW (1982) Substrate utilisation by plant cell cultures. J Chem Tech Biotechnol 32: 338–346
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Pijnacker, L.P., Ferwerda, M.A. Effect of sucrose on polyploidization in early callus cultures of Solanum tuberosum . Plant Cell Tiss Organ Cult 21, 153–157 (1990). https://doi.org/10.1007/BF00033435
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DOI: https://doi.org/10.1007/BF00033435