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Characterization of the photosystem II centers inactive in plastoquinone reduction by fluorescence induction

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Abstract

In order to characterize the photosystem II (PS II) centers which are inactive in plastoquinone reduction, the initial variable fluorescence rise from the non-variable fluorescence level Fo to an intermediate plateau level Fi has been studied. We find that the initial fluorescence rise is a monophasic exponential function of time. Its rate constant is similar to the initial rate of the fastest phase (α-phase) of the fluorescence induction curve from DCMU-poisoned chloroplasts. In addition, the initial fluorescence rise and the α-phase have the following common properties: their rate constants vary linearly with excitation light intensity and their fluorescence yields are lowered by removal of Mg++ from the suspension medium. We suggest that the inactive PS II centers, which give rise to the fluorescence rise from Fo to Fi, belong to the α-type PS II centers. However, since these inactive centers do not display sigmoidicity in fluorescence, they thus do not allow energy transfer between PS II units like PS IIα.

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Abbreviations

DCMU:

3-(3,4-dichlorophenyl)-1,1-dimethyl urea

DMQ:

2,5-dimethyl-p-benzoquinone

Fo :

initial non-variable fluorescence yield

Fm :

maximum fluorescence yield

Fi :

intermediate fluorescence yield

PS II:

photosystem II

QA :

primary quinone acceptor of PS II

QB :

secondary quinone acceptor of PS II

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Authors and Affiliations

  1. Institute of Life Science, National Tsing Hua University, 30043, Hsin-Chu, Taiwan, Republic of China

    Ban-Dar Hsu & Jee-Yau Lee

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  1. Ban-Dar Hsu
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  2. Jee-Yau Lee
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Hsu, BD., Lee, JY. Characterization of the photosystem II centers inactive in plastoquinone reduction by fluorescence induction. Photosynth Res 27, 143–150 (1991). https://doi.org/10.1007/BF00033253

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  • DOI: https://doi.org/10.1007/BF00033253

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