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Resolution of components of non-photochemical chlorophyll fluorescence quenching in barley leaves

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Abstract

Non-photochemical chlorophyll fluorescence quenching (qN) in barley leaves has been analysed by monitoring its relaxation in the dark, by applying saturating pulses of light. At least three kinetically distinct phases to qN recovery are observed, which have previously been identified (Quick and Stitt 1989) as being due to high-energy state quenching (‘fast’), excitation energy redistribution due to a state transition (‘medium’) and photoinhibition (‘slow’). However, measurements of chlorophyll fluorescence at 77 K from leaf extracts show that state transitions only occur in low light conditions, whereas the ‘medium’ component of qN is very large in high light. The source of that part of the ‘medium’ component not accounted for by a state transition is discussed.

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Abbreviations

ATP:

adenosine 5′-triphosphate

DCMU:

3[3,4-dichlorophenyl]-1,1 dimethylurea

ΔpH:

trans-thylakoid pH gradient

Fo, Fm :

room-temperature chlorophyll fluorescence yield with all reaction centres open, closed

Fv :

variable fluorescence = Fm−Fo

LHC II:

Light harvesting complex II

PS I, PS II:

Photosystem I, II

P700, P680:

primary donor in photosystem I, II

qP:

photochemical quenching of variable fluorescence

qN:

non-photochemical quenching of variable fluorescence

qNe, qNt, qNi :

non-photochemical quenching due to high energy state, state transition, photoinhibition

qNf, qNm, qNs :

components of qN relaxing fast, medium, slow

qr :

quenching of r relative to the dark state

tricine:

N-tris[hydroxymethyl]methylglycine

r:

ratio of fluorescence maximum from photosystem II to that from photosystem I at 77 K

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Walters, R.G., Horton, P. Resolution of components of non-photochemical chlorophyll fluorescence quenching in barley leaves. Photosynth Res 27, 121–133 (1991). https://doi.org/10.1007/BF00033251

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