Abstract
The relation between the quantum yield of oxygen evolution of open photosystem II reactions centers (Φp), calculated according to Weis and Berry (1987), and non-photochemical quenching of chlorophyll fluorescence of plants grown at 19°C and 7°C was measured at 19°C and 7°C. The relation was linear when measured at 19°C, but when measured at 7°C a deviation from linearity was observed at high values of non-photochemical quenching. In plants grown at 7°C this deviation occurred at higher values of non-photochemical quenching than in plants grown at 19°C. The deviations at high light intensity and low temperature are ascribed to an increase in an inhibition-related, non-photochemical quenching component (qI).
The relation between the quantum yield of excitation capture of open photosystem II reaction centers (Φexe), calculated according to Genty et al. (1989), and non-photochemical quenching of chlorophyll fluorescence was found to be non-linear and was neither influenced by growth temperature nor by measuring temperature.
At high PFD the efficiency of overall steady state electron transport measured by oxygen-evolution, correlated well with the product of q N and the efficiency of excitation capture (Φexe) but it deviated at low PFD. The deviations at low light intensity are attributed to the different populations of chloroplasts measured by gas exchange and chlorophyll fluorescence and to the light gradient within the leaf.
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Abbreviations
- F0 :
-
basic fluorescence
- F′0 :
-
basic fluorescence, thylakoid in energized state
- Fm :
-
maximal fluorescence
- F′m :
-
maximum fluorescence in energized state
- Fs :
-
steady state fluorescence
- Fv :
-
maximal variable fluorescence
- PFD:
-
photon flux density
- PS IIrc :
-
Photosystem II reaction center
- qF0 :
-
quenching of basic fluorescence
- qE :
-
energy related quenching
- qN :
-
non-photochemical quenching:-qf-total quenching
- qI :
-
‘inhibition’-related quenching
- qp :
-
photochemical quenching
- qr :
-
quenching due to state transition
- Rd :
-
dark respiration
- Φp :
-
PS II efficiency of excitation capture of open PS IIrc
- Φpe :
-
extrapolated minimal value of Φp
- Φp0 :
-
extrapolated maximal value of Φp
- Φsi :
-
quantum efficiency of linear electron transport, calculated from gas exchange measurements based on incident light
- Φsf :
-
quantum efficiency of linear electron transport, calculated from fluorescence measurements, based on incident measuring light
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van Wijk, K.J., van Hasselt, P.R. The quantum efficiency of photosystem II and its relation to non-photochemical quenching of chlorophyll fluorescence; the effect of measuring-and growth temperature. Photosynth Res 25, 233–240 (1990). https://doi.org/10.1007/BF00033164
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DOI: https://doi.org/10.1007/BF00033164