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The effect of high-energy-state excitation quenching on maximum and dark level chlorophyll fluorescence yield

Photosynthesis Research volume 25, pages 199–211 (1990)Cite this article

Abstract

The quenching of variable fluorescence yield (qN) and the quenching of dark level fluorescence yield (q0) directly atributable to high-energy-state fluorescence quenching (qE) was studied to distinguish between energy dissipation in the antenna and light harvesting complexes (antenna quenching) and energy dissipation at the reaction centres (reaction centre quenching). A consistent relationship was obtained between qN and q0 in barley leaves, the green alga Dunaliella C9AA and in pea thylakoids with 2,3,5,6-tetramethyl-p-phenylene diamine (DAD) as mediator of cyclic electron flow around PS 1. This correlated well with the relationship obtained using m-dinitrobenzene (DNB), a chemical model for antenna quenching, to quench fluorescence in Dunaliella C9AA or pea thylakoids. The results also correlated reasonably well with theoretical predictions by the Butler model for antenna quenching, but did not correlate with the predictions for reaction centre quenching. It is postulated that qE quenching therefore occures in the antenna and light harvesting complexes, and that the small deviation from the Butler prediction is due to PS 2 heterogeneity.

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Abbreviations

9-aa:

9-aminoacridine

DCMU:

3-(3′,4′-dichlorophenyl)-1,1-dimethylurea

EDTA:

Ethylenediaminetetra-acetic acid

Hepes:

4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid

Mes:

2-(N-morpholino) prophanesulfonate

PS 1:

photosystem 1

PS 2:

photosystem 2

QA and QB :

primary and secondary stable electron acceptors of photosystem 2

qN :

non-photochemical fluorescence quenching coefficient

qE :

high-energy-state fluorescence quenching coefficient

q0 :

quenching coefficient for F0

F0 :

dark level fluorescence yield

Fm :

maximum fluorescence yield

Fv :

variable fluorescence yield

Fv/Fm :

ratio of variable to total fluorescence yield

DAD:

2,3,5,6-tetramethyl-p-phenylene diamine

DNB:

m-dinitrobenzene

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Authors and Affiliations

  1. Robert Hill Institute, Department of Molecular Biology and Biotechnology, Sheffield University, S10 2TN, Sheffield, UK

    D. Rees, G. D. Noctor & P. Horton

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  1. D. Rees
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  2. G. D. Noctor
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  3. P. Horton
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Rees, D., Noctor, G.D. & Horton, P. The effect of high-energy-state excitation quenching on maximum and dark level chlorophyll fluorescence yield. Photosynth Res 25, 199–211 (1990). https://doi.org/10.1007/BF00033161

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  • DOI: https://doi.org/10.1007/BF00033161

Keywords

  • Reaction Centre
  • Energy Dissipation
  • Chlorophyll Fluorescence
  • Fluorescence Yield
  • Dunaliella