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Chlorophyll luminescence as an indicator of stress-induced damage to the photosynthetic apparatus. Effects of heat-stress in isolated chloroplasts

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Abstract

A brief review is given of investigations on stres-induced alterations of ms-to s-luminescence yield of chlorophyll in plants. Three different approaches are considered: phytoluminography, luminescence-temperature curves, and luminescence induction curves. The remainder of this article presents new results of the effect of heat stress on luminescence induction curves of isolated chloroplasts. Three parameters with widely different heat resistances were resolved from induction curves. A fast valinomycin sensitive transient, L'i, with a 50% inhibition temperature of 33 to 34°C was correlated with the magnitude of the light-induced membrane potential after heat pretreatment. A slower nigericin sensitive transient, L'm, with a 50% inhibition temperature of 39 to 40°C was mainly correlated with the light-induced proton gradient. An uncoupler resistant part of the induction curve, L0, was enhanced by heat stress (half maximum after pretreatment at 46°C) and was correlated with the degree of inhibition of oxygen evolution. Since L0 was also raised by other treatments impairing the oxygen evolving enzyme system, and since this rise was inhibited by DCMU and hydroxylamine, this type of luminescence was ascribed to the intrinsic backreaction. We conclude that luminescence induction curves can serve as an useful indicator of the intactness of the membrane potential, the proton gradient, and the oxygen evolving enzyme system in isolated chloroplasts after heat stress.

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Abbreviations

9-AA:

9-aminoacridine

CCCP:

carbonylcyanide m-chlorophenylhydrazone

ΔA518 :

light-induced absorbance change at 518 nm

ΔAon, ΔAoff :

rapid ΔA518 upon switching actinic light on or off, respectively

Li, Lm, L0 :

in this order, initial spike, main maximum, uncoupler insensitive transient of luminescence induction curve

L'i =:

Li − L0

L'm =:

Lm − L0

P, P680:

primary donor of PS II

PFD:

photon flux density (400–700 nm)

QA :

primary acceptor of PS II

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Author notes

  1. Wolfgang Bilger

    Present address: Department of Plant Biology, Carnegie Institution of Washington, 290 Panama Street, 94305, Stanford, CA, USA

Authors and Affiliations

  1. Institut für Botanik und Pharmazeutische Biologie der Universität Würzburg, Mittlerer Dallenbergweg 64, 87, Würzburg, FRG

    Wolfgang Bilger & Ulrich Schreiber

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  1. Wolfgang Bilger
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  2. Ulrich Schreiber
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Bilger, W., Schreiber, U. Chlorophyll luminescence as an indicator of stress-induced damage to the photosynthetic apparatus. Effects of heat-stress in isolated chloroplasts. Photosynth Res 25, 161–171 (1990). https://doi.org/10.1007/BF00033158

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  • DOI: https://doi.org/10.1007/BF00033158

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