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Dry artificial seeds and desiccation tolerance induction in microspore-derived embryos of broccoli

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Abstract

Desiccation tolerance of broccoli microspore-derived embryos was induced by exogenous application of abscisic acid (ABA). Embryos, which were desiccated to about 10% water content, were estimated for viability after rehydration. Survival was dependent on the ABA concentration and the development stage of embryo, but not on the length of exposure period to ABA or genotype. Cotyledonary stage embryos acquired the highest desiccation tolerance when treated with 1×10-4M ABA. Under this condition, on average 27–48% of the desiccated embryos could convert into plants. Embryos treated with 1×10-6M ABA or no ABA or earlier development-staged embryos, such as globular and heart stages, lost viability after desiccation. A one day exposure to ABA had the similar effect on the induction of desiccation tolerance as a 7-day treatment. The dried embryos maintained their ability of plant conversion after three months of storage under room conditions. The plants derived from the desiccated embryos were not different in the morphology or ploidy level from those from non-desiccated ones.

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Abbreviations

ABA:

abscisic acid

RH:

relative humidity

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Authors and Affiliations

  1. Faculty of Agriculture, Iwate University, 020, Morioka, Japan

    Y. Takahata & N. Kaizuma

  2. Plant Research Centre, Agriculture Canada, K1A 0C6, Ottawa, Canada

    D. C. W. Brown

  3. Plant Biotechnology Institute, National Research Council, S7N 0W9, Saskatoon, Canada

    W. A. Keller

Authors
  1. Y. Takahata
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  2. D. C. W. Brown
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  3. W. A. Keller
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  4. N. Kaizuma
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Takahata, Y., Brown, D.C.W., Keller, W.A. et al. Dry artificial seeds and desiccation tolerance induction in microspore-derived embryos of broccoli. Plant Cell Tiss Organ Cult 35, 121–129 (1993). https://doi.org/10.1007/BF00032961

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  • DOI: https://doi.org/10.1007/BF00032961

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