Abstract
Medium components that may influence the emergence of adventitious root primordia in rhizome buds of Lapageria rosea (Ruiz et Pav.) cv. Nashcourt in vitro were examined. Sucrose was the only medium component tested that affected root emergence. Increasing the sucrose concentration in the medium from 10 to 30 g 1-1 stimulated adventitious root emergence from rhizome buds that had been proliferated on a medium containing the gibberellin biosynthesis inhibitor paclobutrazol (5 μM). Successful clonal propagation of L. rosea cv. Nashcourt can now be achieved by a rhizome bud proliferation stage(s) in the presence of paclobutrazol followed by an adventitious root emergence stage in the absence of paclobutrazol.
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References
Chin C-K (1982) Promotion of shoot and root formation in Asparagus in vitro by ancymidol. HortScience 17: 590–591
Davis TD, Sankhla N, Walser RH & Upadhyaya A (1985) Promotion of adventitious root formation on cuttings by paclobutrazol. HortScience 20: 883–884
Davis TD, Walser RH, Soerensen K & Sankhla N (1986) Rooting and subsequent growth of cuttings treated with paclobutrazol. Plant Propagator 32 (1): 7–9
Desjardins Y (1984) In vitro rooting of nodal sections of Asparagus officinalis L. M.Sc. Thesis, University of Guelph, Canada
Jarvis BC & Booth A (1981) Influence of indolebutyric acid, myo-inositol, vitamin D2 and seedling age on adventitious root development in cuttings of Phaseolus aureus. Physiol. Plant. 53: 213–218
Jarvis BC, Ali AHN & Shaheed AI (1983) Auxin and boron in relation to the rooting response and aging of mung bean cuttings. New Phytol. 95: 509–518
Jordan M, Cortes I & Montenegro G (1983) Regeneration of Lapageria rosea plantlets by tissue culture. (Family Philesiaceae) Gartenbauwissenschaft 48: 97–100
Khosh-Khui M & Sink KC (1982) Micropropagation of New and Old World rose species. J. Hort. Sci. 57: 315–319
Lloyd G & McCown B (1980) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia by use of shoot tip culture. Comb. Proc. Int. Plant Prop. Soc. 30: 421–427
McKinless J (1989) Adventitious rooting in Lapageria and Rosa in vitro. PhD Thesis, University of Nottingham, UK
McKinless J & Alderson PG (1991) An anatomical study of rhizome bud formation induced by paclobutrazol and adventitious root formation in in vitro cultures of Lapageria rosea (Ruiz et Pav.). Ann. Bot. 67: 331–338
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays of tobacco tissue cultures. Physiol. Plant. 15: 473–497
Scherer PA, Muller E, Lippert H & Wolff G (1988) Multielement analysis of agar and Gelrite impurities investigated by inductively coupled plasma emission spectrometry as well as physical properties of tissue culture medium prepared with agar or the gellan gum Gelrite. Acta Hort. 226: 655–658
Seemann FP (1983) Propagation in vitro del copihue (Lapageria rosea Ruiz et Pav.). Agro. Sur. 11: 130–134
Steinitz P, Cohen A, Goldberg Z & Kochba M (1991) Precocious gladiolus corm formation in liquid shake cultures. Plant Cell Tiss. Org. Cult. 26: 63–70
Upadhyaya A, Davis TD & Sankhla N (1986) Some biochemical changes associated with paclobutrazol induced adventitious root formation on bean hypocotyl cuttings. Ann. Bot. 57: 309–315
Wang SY, Byun JK & Steffens GL (1985) Controlling plant growth via the gibberellin pathway. II. Biochemical and physiological alterations in apple seedlings. Physiol. Plant. 63: 169–175
Wang SY & Faust M (1986) The effect of growth retardants on root formation and polyamine content in apple seedlings. J. Amer. Soc. Hort. Sci. 111: 912–917
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McKinless, J., Alderson, P.G. Promotion of root emergence in vitro from rhizome buds of Lapageria rosea cv. Nashcourt after proliferation in the presence of paclobutrazol. Plant Cell Tiss Organ Cult 35, 115–120 (1993). https://doi.org/10.1007/BF00032960
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DOI: https://doi.org/10.1007/BF00032960