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An immunological method for quantitative determination of photosynthetic fructose-1,6-bisphosphatase in leaf crude extracts

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Abstract

An immunological method for quantitative determination of photosynthetic fructose-1,6-bisphosphatase in crude extracts of leaves is proposed. It is based on the ELISA technique, and offers two modifications. A non-competitive technique has a higher sensitivity and is the right option for samples of low fructose-1,6-bisphosphatase content. However, this method is not sufficiently specific when the total protein is higher than 5 μg/cm3; so, despite its lower sensitivity, in these circumstances a competitive technique is more suitable. Thus photosynthetic fructose-1,6-bisphosphatase can be measured without interferences from the gluconeogenic cytosolic enzyme of the photosynthetic cell or from a non-specific phosphatase present in the chloroplast.

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Abbreviations

FBP:

Fructose-1,6-bisphosphate

FBPase:

Fructose-1,6-bisphosphatase

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Authors and Affiliations

  1. Unidad de Bioquímica Vegetal, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008, Granada, Spain

    Rosario Hermoso, Ana Chueca, Juan Jose Lazaro & Julio Lopez-Gorge

Authors
  1. Rosario Hermoso
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  2. Ana Chueca
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  3. Juan Jose Lazaro
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  4. Julio Lopez-Gorge
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Hermoso, R., Chueca, A., Lazaro, J.J. et al. An immunological method for quantitative determination of photosynthetic fructose-1,6-bisphosphatase in leaf crude extracts. Photosynth Res 14, 269–278 (1987). https://doi.org/10.1007/BF00032710

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  • DOI: https://doi.org/10.1007/BF00032710

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