Abstract
We isolated ENOD5, ENOD12 and ENOD40 homologues from Vicia sativa and studied their expression pattern during Rhizobium-induced nodule formation. Comparison of the VsENOD40 nucleotide sequence with the pea, soybean and alfalfa ENOD40 sequences showed that the sequences contain two conserved regions, called region I and region II. Comparison of all the potential open reading frames (ORFs) showed that all the five different ENOD40 clones encode a highly conserved small polypeptide of 12 or 13 amino acids encoded by an ORF located in region I. Furthermore we studied with in situ hybridization the expression pattern of VsENOD5, VsENOD12 and VsENOD40 during Rhizobium-induced nodule formation. Although the expression of these genes is largely similar to that of the pea counterparts, differences where found for the expression of VsENOD12 and VsENOD40 in Vicia. VsENOD12 is expressed in the whole prefixation zone II, whereas in pea ENOD12 is only expressed in the distal part of this zone. VsENOD40 is expressed in the uninfected cells of interzone II–III; while in pea ENOD40 is expressed in both the uninfected and infected cells of this zone.
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References
Asad S, Fang Y, Wycoff KL, Hirsch AM: Isolation and characterization of cDNA and genomic clones of MsE-NOD40; transcripts are detected in meristematic cells of alfalfa. Protoplasma 183: 10–23 (1994).
Brewin NJ: Development of the legume root nodule. Annu Rev Cell Biol 7: 191–226 (1991).
Crespi MD, Jurkevitch E, Poiret M, d'Aubenton-Carafa Y, Petrovics G, Kondorosi E, Kondorosi A: ENOD40, a gene expressed during nodule organogenesis, codes for a non-translatable RNA involved in plant growth. EMBO J 13: 5099–5112 (1994).
Franssen HJ, Vijn I, Yang WC, Bisseling T: Developmental aspects of the Rhizobium-legume symbiosis. Plant Mol Biol 19: 89–107 (1992).
Gubler U: Second-strand cDNA synthesis: mRNA fragments as primers. Meth Enzymol 152: 330–335 (1987).
Heidstra R, Geurts R, Franssen H, Spaink HP, vanKammen A, Bisseling T: Root hair deformation activity of Nod factors and their fate on Vicia sativa. Plant Physiol 105: 787–797 (1994).
Hirsch AM: Developmental biology of legume nodulation. New Phytol 122: 211–237 (1992).
Horvath B, Heidstra R, Lados M, Moerman M, Spaink HP, Promé J-C, vanKammen A, Bisseling T: Lipooligosaccharides of Rhizobium induce infection related early nodulin gene expression in pea root hairs. Plant J 4: 727–733 (1993).
Kouchi H, Hata S: Isolation and characterization of novel nodulin cDNAs representing genes expessed at early stages of soybean nodule development. Mol Gen Genet 238: 106–119 (1993).
Kozak M: Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44: 283–292 (1986).
Krug MS, Berger SL: First-strand cDNA synthesis primed with oligo(dT). Meth of Enzymol 152: 316–325 (1987).
Lutcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheeler GA: Selection of AUG initiation codons differ in plant and animals. EMBO J 6: 43–48 (1987).
Matvienko M, van deSande K, Yang W-C, vanKammen A, Bisseling T, Franssen H: Comparison of soybean and pea ENOD40 cDNA clones representing genes expressed during both early and late stages of nodule development. Plant Mol Biol 26: 487–493 (1994).
Pawlowski K, Kunze R, deVries S, Bisseling T: Isolation of total, poly(A) and polysomal RNA from plant tissues. In: Gelvin SB, Schilperoort RA (eds) Plant Molecular Biology Manual, pp. D5: 1–13. Kluwer Academic Publishers, Dordrecht (1994).
Scheres B, van deWiel C, Zalensky A, Horvath B, Spaink H, VanEck H, Zwartkruis F, Wolters AM, Gloudemans T, vanKammen A, Bisseling T: The ENOD12 gene product is involved in the infection process during pea-Rhizobium interaction. Cell 60: 281–294 (1990).
Scheres B, vanEngelen F, van derKnaap E, van deWiel C, vanKammen A, Bisseling T: Sequential induction of nodulin gene expression in the developing pea nodule. Plant Cell: 8: 687–700 (1990).
Showalter AM: Structure and function of plant cell wall proteins. Plant Cell 5: 9–23 (1993).
Spaink HP, Sheeley DM, vanBrussel AAN, Glushka J, York WS, Tak T, Geiger O, Kennedy EP, Reinhold VN, Lugtenberg BJJ: A novel highly unsaturated fatty acid moiety of lipo-oligosaccharide signals determines host specificity of Rhizobium. Nature 354: 125–130 (1991).
Stokkermans TJW, Peters NK: Bradyrhizobium elkanii lipo-oligosaccharide signals induce complete nodule structures on Glycine soja Siebold et Zucc. Planta 193: 413–420 (1994).
Truchet G, Roche P, Lerouge P, Vasse J, Camut S, deBilly F, Promé J-C, Dénarié J: Sulphated lipo-oligosaccharide signals of Rhizobium meliloti elicit root nodule organogenesis in alfalfa. Nature 351: 670–673 (1991).
Vasse J, DeBilly F, Camut S, Truchet G: Correlation between ultrastructural differentiation of bacteriods and nitrogen fixation in alfalfa nodules. Bact J 172: 4295–4306 (1990).
Vijn I, Christiansen H, Lauridsen P, Kardailsky I, Quandt H-J, Drenth J, Østergaard Jensen E, van Kammen A, Bisseling T: A 200 bp region of the pea ENOD12 promoter is sufficient for nodule specific and Nod factor-induced expression. Plant Mol Biol, submitted (1995).
Yang W-C, deBlank C, Meskiene I, Hirt H, Bakker J, vanKammen A, Franssen H, Bisseling T: Rhizobium Nod factors reactivate the cell cycle during infection and nodule primordium formation, but the cycle is only completed in primordium formation. Plant Cell 6: 1415–1426 (1994).
Yang WC, Katinakis P, Hendriks P, Smolders A, deVries F, Spee J, vanKammen A, Bisseling T, Franssen H: Characterization of GmENOD40, a gene showing novel patterns of cell-specific expression during soybean nodule development. Plant J 3: 573–585 (1993).
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Vijn, I., Yang, WC., Pallisgård, N. et al. VsENOD5, VsENOD12 and VsENOD40 expression during Rhizobium-induced nodule formation on Vicia sativa roots. Plant Mol Biol 28, 1111–1119 (1995). https://doi.org/10.1007/BF00032671
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DOI: https://doi.org/10.1007/BF00032671