Non-photochemical quenching of chlorophyll fluorescence in the green alga Dunaliella

Abstract

The relaxation of the non-photochemical quenching of chlorophyll fluorescence has been investigated in cells of the green alga Dunaliella following illumination. The relaxation after the addition of DCMU or darkening was strongly biphasic. The uncoupler NH4Cl induced rapid relaxation of both phases, which were therefore both energy-dependent quenching, qE. The proportion of the slow phase of qE increased at increasing light intensity. In the presence of the inhibitors rotenone and antimycin the slow phase of qE was stabilised for in excess of 15 min. NaN3 inhibited the relaxation of almost all the qE. The implications of these results are discussed in terms of the interpretation of the non-photochemical quenching of chlorophyll fluorescence in vivo and the mechanism of qE.

This is a preview of subscription content, access via your institution.

Abbreviations

PS II:

Photosystem II

qQ:

photochemical quenching of chlorophyll fluorescence

qNP:

non-photochemical quenching of chlorophyll fluorescence

qE:

energy-dependent quenching of chlorophyll fluorescence

F m :

maximum level of chlorophyll fluorescence for dark adapted cells

F′ m :

level of fluorescence at any time when qQ is zero

References

  1. Bradbury M and Baker NR (1981) Analysis of the slow phases of the in vivo chlorophyll fluorescence induction curve. Changes in the redox state of Photosystem II electron acceptors and fluorescence emission from Photosystems I and II. Biochim Biophys Acta 635: 542–551

    PubMed  Google Scholar 

  2. Briantais J-M, Vernotte C, Picaud M and Krause GH (1979) A quantitative study of the slow decline of chlorophyll a fluorescence in isolated chloroplasts. Biochim Biophys Acta 548: 128–138

    PubMed  Google Scholar 

  3. Cleland RE, Melis A and Neale PJ (1986) Mechanisms of photoinhibition: photochemical reaction centre inactivation in system II of chloroplasts. Photosyn Res 9: 79–88

    Google Scholar 

  4. Demmig B, Winter K, Kruger A and Czygan F-C (1987) Photoinhibition and zeaxanthin formation in intact leaves. Plant Physiol 84: 218–224

    Google Scholar 

  5. Demmig-Adams B, Winter K, Kruger A and Czygan F-C (1989) Zeaxanthin and the induction and relaxation kinetics of the dissipation of excess excitation energy in leaves in 2% O2, 0% CO2. Plant Physiol 90: 881–886

    Google Scholar 

  6. Dietz KJ and Heber U (1986) Light and CO2 limitation of photosynthesis and states of the reactions regenerating ribulose-1,5 bisphosphate and reducing 3-phosphoglycerate. Biochim Biophys Acta 846: 392–404

    Google Scholar 

  7. Gilmour DJ, Hipkins MF and Boney AD (1982) The effect of salt stress on the primary processes of photosynthesis in Dunaliella tertiolecta. Plant Sci Lett 26: 325–330

    Google Scholar 

  8. Horton P and Black MT (1981) Light-dependent quenching of chlorophyll fluorescence in pea chloroplasts induced by adenosine 5′-triphosphate. Biochim Biophys Acta 635: 53–62

    PubMed  Google Scholar 

  9. Horton P, Crofts J, Gordon S, Oxborough K, Rees D and Scholes JD (1989) Regulation of Photosystem II by metabolic and environmental factors. Phil Trans R Soc Lond B323: 269–279

    Google Scholar 

  10. Horton P and Hague A (1988) Studies on the induction of chlorophyll fluorescence in isolated barley protoplasts. IV Resolution of non-photochemical quenching. Biochim Biophys Acta 932: 107–115

    Google Scholar 

  11. Horton P, Oxborough K, Rees D and Scholes JD (1988) Regulation of the photochemical efficiency of photosystem II: consequences for the light response of field photosynthesis. Plant Physiol Biochem 26: 453–460

    Google Scholar 

  12. Krause GH, Laasch H and Weis E (1988) Regulation of thermal dissipation of absorbed light energy in chloroplasts indicated by energy-dependent fluorescence quenching. Plant Physiol Biochem 26: 445–452

    Google Scholar 

  13. Krause GH, Vernotte C and Briantais J-M (1982) Photoinduced quenching of chlorophyll fluorescence in intact chloroplasts and algae. Resolution into two components. Biochim Biophys Acta 679: 116–124

    Google Scholar 

  14. Minkov IV and Strotmann H (1989) The effect of azide on regulation of the chloroplast H+-ATPase by ADP and phosphate. Biochim Biophys Acta 973: 7–12

    Google Scholar 

  15. Oxborough K and Horton P (1987) Characterization of the effects of antimycin A upon high energy state quenching of chlorophyll fluorescence in spinach and pea chloroplasts. Photosyn Res 12: 119–128

    Google Scholar 

  16. Rees D, Young A, Noctor G, Britton G and Horton P (1989) Enhancement of the ΔpH dependent dissipation of excitation energy by the presence of zeaxanthin. FEBS Lett 256: 85–90

    Article  Google Scholar 

  17. Walker DA (1981) Secondary fluorescence kinetics of spinach leaves in relation to the onset of photosynthetic carbon assimilation. Planta 153: 273–278

    Google Scholar 

  18. Walters R and Horton P (1989) The use of light pulses to investigate the relaxation in the dark of chlorophyll quenching in barley leaves. In: Baltscheffsky (ed) Proc VIII Int Cong Photosyn. Kluwer, Dordrecht, Netherlands (in press)

    Google Scholar 

  19. Weis E and Berry JA (1987) Quantum efficiency of PS 2 in relation to “energy” dependent quenching of chlorophyll fluorescence. Biochim Biophys Acta 894: 198–208

    Google Scholar 

  20. Weis E and Lechtenberg D (1989) Fluorescence analysis during steady-state photosynthesis. Phil Trans R Soc Lond B323: 253–268

    Google Scholar 

  21. Yamamoto HY (1979) Biochemistry of the violaxanthin cycle in higher plants. Pure and Appl Chem 51: 639–648

    Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lee, C.B., Rees, D. & Horton, P. Non-photochemical quenching of chlorophyll fluorescence in the green alga Dunaliella . Photosynth Res 24, 167–173 (1990). https://doi.org/10.1007/BF00032596

Download citation

Key words

  • chlorophyll fluorescence
  • photosynthesis
  • Dunaliella