Abstract
The relaxation of the non-photochemical quenching of chlorophyll fluorescence has been investigated in cells of the green alga Dunaliella following illumination. The relaxation after the addition of DCMU or darkening was strongly biphasic. The uncoupler NH4Cl induced rapid relaxation of both phases, which were therefore both energy-dependent quenching, qE. The proportion of the slow phase of qE increased at increasing light intensity. In the presence of the inhibitors rotenone and antimycin the slow phase of qE was stabilised for in excess of 15 min. NaN3 inhibited the relaxation of almost all the qE. The implications of these results are discussed in terms of the interpretation of the non-photochemical quenching of chlorophyll fluorescence in vivo and the mechanism of qE.
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Abbreviations
- PS II:
-
Photosystem II
- qQ:
-
photochemical quenching of chlorophyll fluorescence
- qNP:
-
non-photochemical quenching of chlorophyll fluorescence
- qE:
-
energy-dependent quenching of chlorophyll fluorescence
- F m :
-
maximum level of chlorophyll fluorescence for dark adapted cells
- F′ m :
-
level of fluorescence at any time when qQ is zero
References
Bradbury M and Baker NR (1981) Analysis of the slow phases of the in vivo chlorophyll fluorescence induction curve. Changes in the redox state of Photosystem II electron acceptors and fluorescence emission from Photosystems I and II. Biochim Biophys Acta 635: 542–551
Briantais J-M, Vernotte C, Picaud M and Krause GH (1979) A quantitative study of the slow decline of chlorophyll a fluorescence in isolated chloroplasts. Biochim Biophys Acta 548: 128–138
Cleland RE, Melis A and Neale PJ (1986) Mechanisms of photoinhibition: photochemical reaction centre inactivation in system II of chloroplasts. Photosyn Res 9: 79–88
Demmig B, Winter K, Kruger A and Czygan F-C (1987) Photoinhibition and zeaxanthin formation in intact leaves. Plant Physiol 84: 218–224
Demmig-Adams B, Winter K, Kruger A and Czygan F-C (1989) Zeaxanthin and the induction and relaxation kinetics of the dissipation of excess excitation energy in leaves in 2% O2, 0% CO2. Plant Physiol 90: 881–886
Dietz KJ and Heber U (1986) Light and CO2 limitation of photosynthesis and states of the reactions regenerating ribulose-1,5 bisphosphate and reducing 3-phosphoglycerate. Biochim Biophys Acta 846: 392–404
Gilmour DJ, Hipkins MF and Boney AD (1982) The effect of salt stress on the primary processes of photosynthesis in Dunaliella tertiolecta. Plant Sci Lett 26: 325–330
Horton P and Black MT (1981) Light-dependent quenching of chlorophyll fluorescence in pea chloroplasts induced by adenosine 5′-triphosphate. Biochim Biophys Acta 635: 53–62
Horton P, Crofts J, Gordon S, Oxborough K, Rees D and Scholes JD (1989) Regulation of Photosystem II by metabolic and environmental factors. Phil Trans R Soc Lond B323: 269–279
Horton P and Hague A (1988) Studies on the induction of chlorophyll fluorescence in isolated barley protoplasts. IV Resolution of non-photochemical quenching. Biochim Biophys Acta 932: 107–115
Horton P, Oxborough K, Rees D and Scholes JD (1988) Regulation of the photochemical efficiency of photosystem II: consequences for the light response of field photosynthesis. Plant Physiol Biochem 26: 453–460
Krause GH, Laasch H and Weis E (1988) Regulation of thermal dissipation of absorbed light energy in chloroplasts indicated by energy-dependent fluorescence quenching. Plant Physiol Biochem 26: 445–452
Krause GH, Vernotte C and Briantais J-M (1982) Photoinduced quenching of chlorophyll fluorescence in intact chloroplasts and algae. Resolution into two components. Biochim Biophys Acta 679: 116–124
Minkov IV and Strotmann H (1989) The effect of azide on regulation of the chloroplast H+-ATPase by ADP and phosphate. Biochim Biophys Acta 973: 7–12
Oxborough K and Horton P (1987) Characterization of the effects of antimycin A upon high energy state quenching of chlorophyll fluorescence in spinach and pea chloroplasts. Photosyn Res 12: 119–128
Rees D, Young A, Noctor G, Britton G and Horton P (1989) Enhancement of the ΔpH dependent dissipation of excitation energy by the presence of zeaxanthin. FEBS Lett 256: 85–90
Walker DA (1981) Secondary fluorescence kinetics of spinach leaves in relation to the onset of photosynthetic carbon assimilation. Planta 153: 273–278
Walters R and Horton P (1989) The use of light pulses to investigate the relaxation in the dark of chlorophyll quenching in barley leaves. In: Baltscheffsky (ed) Proc VIII Int Cong Photosyn. Kluwer, Dordrecht, Netherlands (in press)
Weis E and Berry JA (1987) Quantum efficiency of PS 2 in relation to “energy” dependent quenching of chlorophyll fluorescence. Biochim Biophys Acta 894: 198–208
Weis E and Lechtenberg D (1989) Fluorescence analysis during steady-state photosynthesis. Phil Trans R Soc Lond B323: 253–268
Yamamoto HY (1979) Biochemistry of the violaxanthin cycle in higher plants. Pure and Appl Chem 51: 639–648
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Lee, C.B., Rees, D. & Horton, P. Non-photochemical quenching of chlorophyll fluorescence in the green alga Dunaliella . Photosynth Res 24, 167–173 (1990). https://doi.org/10.1007/BF00032596
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DOI: https://doi.org/10.1007/BF00032596
Key words
- chlorophyll fluorescence
- photosynthesis
- Dunaliella