Skip to main content
Log in

Expression of alcohol dehydrogenase in rice embryos under anoxia

  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Summary

Alcohol dehydrogenase (ADH) activity was present in roots and shoots of 48-h rice embryos and rose in response to anoxia. The increase was accompanied by changes in the ADH isozyme pattern. Translatable levels of mRNA for two ADH peptides increases as early as 1 h after the beginning of anoxic treatment. Adh mRNA was detected in aerobically grown rice embryos by hybridization to maize Adh1 cDNA: its level increased significantly after 3 h of anoxia.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Al-Ani A, Leblanc JM, Raymond P, Pradet A: Effet de la pression partielle d'oxygène sur la vitesse de germination des semences à réserves lipidiques et amylacées: rôle du métabolisme fermentaire. C.R. Acad Sci Paris 295:271–274, 1982.

    Google Scholar 

  2. Aspart L, Got A, Delseny M, Mocquot B, Pradet A: Adaptation of ribonucleic acid metabolism to anoxia in rice embryos. Plant Physiol 72:115–121, 1983.

    Google Scholar 

  3. Bertani A, Menegus F, Bollini R: Some effects of anaerobiosis on protein metabolism in rice roots. Z Pflanzenphysiol 103:37–43, 1981.

    Google Scholar 

  4. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein binding. Analyt Biochem 72:248–254 (1976).

    Google Scholar 

  5. Dennis ES, Gerlach WL, Pryor AJ, Bennetzen JL, Ingles A, Llewellyn D, Sachs MM, Ferl RJ, Peacock WJ: Molecular analysis of the alcohol dehydrogenase (adh 1) gene of maize. Nucleic Acids Res 12:3983–4000, 1984.

    Google Scholar 

  6. Dennis ES, Sachs MM, Gerlach WL, Finnegan EJ, Peacock WJ: Molecular analysis of the alcohol dehydrogenase 2 (Adh2) gene of maize. Nucleic Acids Res 13:727–743, 1985.

    Google Scholar 

  7. Endo T, Morishma H: Rice. In: Tanksley SD, Orton TJ (eds) Isozymes in Plant Genetics and Breeding, Part B. Elsevier Science Publishers B.V., Amsterdam, 1983, pp 129–146.

    Google Scholar 

  8. Ferl RJ, Dlouhy SR, Schwartz D: Analysis of maize alcohol dehydrogenase by native SDS two dimensional electrophoresis and autoradiography. Mol Gen Genet 169:7–12, 1979.

    Google Scholar 

  9. Ferl RJ, Brennan MD, Schwartz D: In vitro translation of maize ADH. Evidence for the anaerobic induction of mRNA. Biochem Genet 7/8:681–691, 1980.

    Google Scholar 

  10. Gerlach WL, Pryor AJ, Dennis ES, Ferl RJ, Sachs MM, Peacock WJ: cDNA cloning and induction of the alcohol dehydrogenase gene (Adh1) of maize. Proc Natl Acad Sci USA 79:2981–2985, 1982.

    Google Scholar 

  11. Hanson AD, Jacobsen JV, Zwar JA: Regulated expression of three alcohol dehydrogenase genes in barley aleurone layers. Plant Physiol 75:573–581, 1984.

    Google Scholar 

  12. Kelly J, Freeling M: Purification of maize alcohol dehydrogenase 1 allozymes and comparison of their tryptic peptides. Biochimica et Biophysica Acta 624:102–110, 1980.

    Google Scholar 

  13. Kelley PM, Freeling M: Anaerobic expression of maize glucose phosphate isomerase I. J Biol Chem 259:673–677, 1984.

    Google Scholar 

  14. Kelley PM, Freeling M: Anaerobic expression of maize fructose 1,6-diphosphate aldolase. J Biol Chem 259: 14180–14183, 1984.

    Google Scholar 

  15. Laroche-Raynal M, Aspart L, Delseny M, Penon P: Characterization of radish mRNA at three development stages. Plant Sci Lett 35:139–146, 1984.

    Google Scholar 

  16. Laszlo A, St. Lawrence P: Parallel induction and synthesis of PDC and ADH in anoxic maize roots. Mol Gen Genet 192:110–117, 1983.

    Google Scholar 

  17. Maniatis T, Fritsch EF, Sambrook J: Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, 1982.

  18. Mocquot B, Pradet A, Litvak S: DNA synthesis and anoxia in rice coleoptiles. Plant Sci Lett 9:365–371, 1977.

    Google Scholar 

  19. Mocquot B, Prat C, Mouches C, Pradet A: Effect of anoxia on energy charge and protein synthesis in rice embryo. Plant Physiol 68:636–640, 1981.

    Google Scholar 

  20. Parnes JR, Velan B, Felsenfeld A, Ramanathan L, Ferrini U, Appella E, Seidman JG: Mouse ß2-micro globulin cDNA clones: a screening procedure for cDNA clones corresponding to rare mRNAs. Proc Natl Acad Sci USA 78:2253–2257, 1981.

    Google Scholar 

  21. Raymond P, Al-Ani A, Pradet A: Low contribution of non-respiratory pathways in ATP regeneration during early germination of lettuce seeds. Physiol Vég 21:677–687, 1983.

    Google Scholar 

  22. Sachs MM, Freeling M: Selective synthesis of alcohol dehydrogenase during anaerobic treatment of maize. Mol Gen Genet 161:111–115, 1978.

    Google Scholar 

  23. Sachs MM, Freeling M, Okimoto R: The anaerobic proteins of maize. Cell 20:761–767, 1980.

    Google Scholar 

  24. Second G, Trouslot P: Electrophorèse d'énzymes de riz (Oryza sp.), Travaux et Documents de l'ORSTOM no 120, ORSTOM, Paris, 1980.

    Google Scholar 

  25. Shimomura S, Beevers H: Alcohol dehydrogenase and an inactivator from rice seedlings. Plant Physiol 71:736–741, 1983.

    Google Scholar 

  26. Werner D, Chemla Y, Herzberg M: Isolation of poly (A) + RNA by paper affinity chromatography. Analyt Biochem 141:329–336, 1984.

    Google Scholar 

  27. Yatazawa M, Furuhashi K, Shimizu M: Growth of callus tissue from rice-root in vitro. Plant Cell Physiol 8:363–373, 1967.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ricard, B., Mocquot, B., Fournier, A. et al. Expression of alcohol dehydrogenase in rice embryos under anoxia. Plant Mol Biol 7, 321–329 (1986). https://doi.org/10.1007/BF00032562

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00032562

Keywords

Navigation