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Reconstitution of electron transport by cytochrome c-553 in a cell-free system of Nostoc muscorum

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Abstract

Treatment of spheroplasts of Nostoc museorum with hypotonic buffer results in membranes depleted of cytochrome c-553, but still active in photosynthetic and respiratory electron transport. These membranes retain full photosystem II activity (H2O→DADox). Complete linear electron transport (H2O→NADP+), however, is decreased as compared with untreated spheroplasts. Addition of basic Nostoc cytochrome c-553 to depleted membranes reconstitutes NADP+ reduction and redox reactions of the photosystem I region as well.

Using NADPH as electron donor, respiration of depleted membranes is also stimulated by adding cytochrome c-553, indicative of its function in respiratory electron transport.

Cytochrome c-553 from Bumilleriopsis filiformis, Spirulina platensis (acidic types), Phormidium foveolarum (basic type), and mitochondrial horse-heart cytochrome c-550 are not effective in reconstituting both photosynthetic and respiratory electron transport, which points to a specific role of Nostoc cytochrome c-553.

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Abbreviations

BSA:

bovine serum albumin

DAD:

3,6-diaminodurene

DADox :

3,6-diaminodurene oxidized by potassium ferricyanide

DBMIB:

2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone

DCMU:

3-(3,4-dichlorophenyl)-1,1-dimethylurea

DCIP:

2,6-dichlorophenolindophenol

DPC:

1,5-diphenylcarbazide

Fd:

ferredoxin

HEPES:

N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid

MES:

2(-N-morpholino)-ethanesulfonic acid

MV:

methylviologen (1,1′-dimethyl-4,4′-bipyridylium dichloride)

PS I:

photosystem I

PS II:

photosystem II

Tris:

tris-(hydroxymethyl)-aminomethane

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Authors and Affiliations

  1. Lehrstuhl für Physiologie und Biochemic der Pflanzon, Universität Konstanz, D-7750, Konstanz, Germany

    Erwin Stürzl, Siegfried Scherer & Peter Böger

Authors
  1. Erwin Stürzl
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  2. Siegfried Scherer
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  3. Peter Böger
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Stürzl, E., Scherer, S. & Böger, P. Reconstitution of electron transport by cytochrome c-553 in a cell-free system of Nostoc muscorum . Photosynth Res 3, 191–201 (1982). https://doi.org/10.1007/BF00032256

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  • DOI: https://doi.org/10.1007/BF00032256

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