Summary
The use of soil. naturally infested with Heterodera avenae, to select resistant heterozygotes in backcross progenies of wheat, was tested for reliability. Selfed progenies from plants selected as resistant were cultured monoxenically in test tubes with nematodes hatched from single cysts, while backcross progenies from the same parent plants were grown in pots of naturally infested soil. Cyst counts were made after two months' growth. The results showed that over 50% of the backcross lines, screened in previous generations with naturally infested soil, had been erroneously selected as resistant. The test tube cultures clearly differentiated lines carrying resistance from those which were susceptible and corroborated results from pot tests.
Similar content being viewed by others
References
Brown, J. A. M., 1974. Test tube reproduction of Heterodera avenae on resistant and suseeptible wheats. Nematologica 20: 192–203.
Brown, J. A. M. & S. E., Ellis, 1976. Breeding for resistance to cereal cyst nematode in wheat. Euphytica 25: 73–82.
Brown, J. A. M. & W. H., Klein, 1971. Photomorphogenesis in Arabidopsis thaliana (L.) Heynh. Pl. Physiol., Lancaster 47: 393–399.
Brown, R. H. & J. W., Meagher, 1970. Resistance in cereals to the cyst nematode (Heterodera avenae Woll.) in Victoria. Aust. J. esp. Agric. Anim. Husb. 10: 360–365.
Meagher, J. W. & S. C., Chambers, 1971. Pathogenic effects of Heterodera avenae and Rhizoctonia solani and their interaction on wheat. Aust. J. agric. Res. 22: 189–194.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Brown, J.A.M. Breeding for resistance to cereal cyst nematode in wheat II. Use of test tube cultures in selection. Euphytica 26, 89–95 (1977). https://doi.org/10.1007/BF00032073
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00032073