Abstract
Genotypes representing the three botanical varieties of peanut (Arachis hypogaea L.) were assessed for somatic embryogenesis and subsequent plant conversion from mature zygotic embryo axes. Explants were initially cultured on Murashige and Skoog medium supplemented with 12.42 μM 4-amino-3,5,6-trichloropicolinic acid. Individual somatic embryos wer isolated from explant tissue and used to initiate repetitive liquid cultures. There were significant differences among genotypes and varieties for somatic embryo formation and plant regeneration using a single media sequence. Botanical variety fastigiata had a lower embryogenic frequency and produced significantly fewer embryos than either hypogaea or vulgaris, which were similar in response.
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Abbreviations
- EA:
-
zygotic embryo axes
- MS:
-
Murashige and Skoog (1962) medium
- picloram:
-
4-amino-3,5
- 6:
-
trichloropicolinic acid
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McKently, A.H. Effect of genotype on somatic embryogenesis from axes of mature peanut embryos. Plant Cell Tiss Organ Cult 42, 251–254 (1995). https://doi.org/10.1007/BF00029995
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DOI: https://doi.org/10.1007/BF00029995