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Plant Cell, Tissue and Organ Culture

, Volume 42, Issue 3, pp 245–250 | Cite as

Improved isolated microspore culture efficiency in medium with maltose and optimized growth regulator combination in japonica rice (Oryza sativa)

  • Jiahua Xie
  • Mingwei Gao
  • Qihua Cai
  • Xiongying Cheng
  • Yuwei Shen
  • Zhuqing Liang
Original Research Papers

Abstract

The influence of maltose and growth regulators on microspore culture response was investigated in japonica rice. High frequency of callus induction of isolated microspores was obtained with liquid medium containing MS salts, 100 mg l−1 myo-inositol, 1 mg l−1 thiamine-HCl, 500 mg l−1 glutamine, 60 g l−1 maltose, and several growth regulators. The effect of maltose on promoting callus formation was associated with keeping a high proportion of swollen microspores after 5 day preculture and increasing the microspore division rate on the 3rd day after culture initiation. No significant effect of maltose in place of sucrose on plantlet regeneration was seen in regeneration medium. Among the growth regulators tested, the combination of auxin 2,4-dichlorophenoxyacetic acid (1 mg l−1), naphthaleneacetic acid (1 mg l−1), and cytokinin (6-benzyl-aminopurine 1 mg l−1) in the medium proved to be much better for callus formation than in the other media, and the percentage of callusing microspores of that medium reached 0.86%. Indole-3-acetic acid (0.5 mg l−1) and kinetin (2 mg l−1) in regeneration medium were beneficial for green plantlet differentiation. The results also showed that the frequencies of microspores initial division, callus formation and green plant regeneration varied among genotypes no matter what kind of growth regulator and sugar were used. Xiushui 117 was the best variety for callusing followed by 02428 & Taipei 309. Taipei 309 showed a good ability for green plantlet regeneration.

Key words

androgenesis microspore culture Oryza sativa L. plant regeneration 

Abbreviations

2,4-d

2,4-dichlorophenoxyacetic acid

NAA

naphthaleneacetic acid

6-BA

6-benzylaminopurine

KT

kinetin

IAA

indole-3 acetic acid

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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Jiahua Xie
    • 1
  • Mingwei Gao
    • 1
  • Qihua Cai
    • 1
  • Xiongying Cheng
    • 1
  • Yuwei Shen
    • 1
  • Zhuqing Liang
    • 1
  1. 1.Institute of Nuclear Agricultural SciencesZhejiang Agricultural UniversityHangzhouChina

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